Description

Cy5 (Di So3,Di Et) CAS NO 888014-73-1 is a sulfonated, ethylated derivative of the cyanine dye Cy5, engineered for enhanced water solubility and stability. This modification is critical for applications requiring minimal non-specific binding and high signal-to-noise ratios in aqueous biological matrices. It is an essential reagent for researchers and manufacturers in the life sciences and diagnostic industries who require reliable, high-performance fluorescent labeling.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and quantification.
• Nucleic Acid Analysis: Labeling primers and probes for real-time PCR (qPCR), fluorescence in situ hybridization (FISH), and microarray applications.
• Bioimaging and Microscopy: Cell labeling, receptor tracking, and in vivo imaging due to its near-infrared emission, which reduces background autofluorescence.
• Diagnostic Assay Development: Key component in lateral flow assays, ELISA, and other immunoassays requiring stable, bright fluorescent signals.
• Flow Cytometry: Used for cell surface and intracellular marker staining, enabling multiparameter analysis.
• Material Science: Incorporation into polymers and nanoparticles for creating functional fluorescent materials and sensors.

Basic Information

Product Name	Cy5 (Di So3,Di Et)
CAS No.	888014-73-1
Molecular Formula	C37H45N2O9S2+
Molecular Weight	~725.9 g/mol (as cation)
Synonyms	Sulfo-Cy5 diethyl; Cy5 Diethyl Sulfonate; 1,1'-(1E,1'E)-3,3'-(Ethane-1,2-diyl)bis(1-ethyl-3,3-dimethyl-5-sulfoindoline-2-ylidene)prop-1-ene-1,3-diyl)bis(ethane-1,1-diyl) diethyl sulfate; Cy5 NHS ester sulfonated derivative; Water-soluble Cy5 dye; Cy5 bis-sulfonate ethyl ester; Near-IR fluorescent dye Cy5-SE analog; Cy5 alky sulfonate
EINECS	Contact for details

Quality Control

Every batch of Cy5 (Di So3,Di Et) is manufactured and tested under a strict quality management system. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, identity, and performance characteristics is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect performance. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Extinction Coefficient	≥ 250,000 cm⁻¹M⁻¹ (at λmax ~649 nm)
Fluorescence Emission	~670 nm
Solubility	Freely soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.