Description

o-Methyl-o-(n-Butylfluorescein)Phosphate is a specialized organophosphate derivative of fluorescein, designed for advanced biochemical and analytical applications. This compound is valued for its role as a highly sensitive fluorogenic substrate for detecting enzymatic activity, particularly of phosphatases and esterases. It is essential for researchers and manufacturers in the fields of diagnostic assay development, high-throughput screening (HTS), and biochemical research, where precise and sensitive detection is paramount.

Application

• Fluorogenic Substrate for Phosphatase Assays: Primary use in enzymatic assays to detect and quantify alkaline phosphatase (ALP) and acid phosphatase activity with high sensitivity.
• Diagnostic Kit Manufacturing: A key component in the development of clinical diagnostic kits for various diseases, including liver and bone disorders.
• High-Throughput Screening (HTS): Used in pharmaceutical and biotechnology research for drug discovery campaigns targeting phosphatase enzymes.
• Cell-Based Imaging and Reporter Assays: Enables visualization and measurement of intracellular enzymatic activity in live or fixed cells.
• Biosensor Development: Incorporated into biosensor platforms for environmental monitoring or point-of-care testing devices.
• Biochemical Research Reagent: Serves as a critical tool for studying enzyme kinetics, inhibition, and signal transduction pathways.

Basic Information

Product Name	o-Methyl-o-(n-Butylfluorescein)Phosphate
CAS No.	887406-94-2
Molecular Formula	C29H29O8P
Molecular Weight	536.51 g/mol
Synonyms	o-Methyl-o-(n-Butylfluorescein) Phosphate; Butylfluorescein Methyl Phosphate; Fluorescein Dibutyl Phosphate Methyl Ester; Fluorogenic Phosphatase Substrate; ALP Substrate (Fluorogenic); 3',6'-Bis(butyloxy)-3H-spiro[isobenzofuran-1,9'-xanthene]-3-one, 2-(phosphonooxy)methyl ester; Phosphoric Acid Mono[(3-oxo-6-pentyloxy-3H-xanthen-9-yl)benzoate] Methyl Ester
EINECS	Contact for details

Quality Control

Our o-Methyl-o-(n-Butylfluorescein)Phosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (Fluorimetric)	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.