Description

Cyclooctyne-o-Pfp Ester is a highly reactive, strained alkyne derivative specifically designed for bioorthogonal click chemistry applications. Its core value lies in enabling rapid, selective, and catalyst-free conjugation with azides under physiological conditions, a critical requirement for advanced biological research and development. This compound is essential for researchers and manufacturers in pharmaceutical development, bioconjugation, proteomics, and materials science who require efficient and reliable labeling and crosslinking strategies.

Application

• Bioorthogonal Labeling: Selective tagging of biomolecules (proteins, nucleic acids, glycans) in live cells and organisms via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Bioconjugation Reagent: Site-specific modification of antibodies, peptides, and other therapeutic proteins for the development of antibody-drug conjugates (ADCs) and diagnostics.
• Chemical Proteomics: Activity-based protein profiling (ABPP) and identification of enzyme targets using probe molecules.
• Polymer and Materials Functionalization: Surface modification of nanoparticles, hydrogels, and polymers to introduce bioactive ligands or alter material properties.
• Fluorescent Probe Synthesis: Precursor for constructing turn-on or turn-off fluorescent sensors for cellular imaging and detection assays.
• Glycan Imaging and Analysis: Metabolic labeling and visualization of cell-surface glycans for studying glycosylation in disease states.

Basic Information

Product Name	Cyclooctyne-o-Pfp Ester
CAS No.	886209-60-5
Molecular Formula	C₁₉H₁₀F₅NO₃
Molecular Weight	395.28 g/mol
Synonyms	Cyclooctyne Pentafluorophenyl Ester; 3,3,4,4,5,5,6,6-Octafluoro-8-oxa-2,9-diazabicyclo[6.1.0]non-4-ene-9-carboxylic acid 2,3,4,5,6-pentafluorophenyl ester; BCN-PFP ester; Bicyclo[6.1.0]non-4-yne-9-carboxylic acid, 3,3,4,4,5,5,6,6-octafluoro-8-oxa-2,9-diazabicyclo[6.1.0]non-4-ene-9-carboxylic acid 2,3,4,5,6-pentafluorophenyl ester; BCN-PFP; BCN-O-Pfp; BCN Active Ester; Dibenzocyclooctyne-PFP Ester (common misnomer).
EINECS	Contact for details

Quality Control

Our Cyclooctyne-o-Pfp Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high reactivity. Each lot is characterized by multiple analytical techniques to confirm identity, purity, and suitability for sensitive bioorthogonal applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity confirmation (NMR, IR), and moisture content is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dedicated freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.