Description

23-(Dipyrrometheneboron Difluoride)-24-Norcholesterol is a sophisticated fluorescently labeled sterol derivative, designed for advanced research and diagnostic applications. This compound is engineered to combine the structural framework of cholesterol with a highly photostable and bright BODIPY (boron-dipyrromethene) fluorophore, enabling precise cellular and molecular tracking. It is an essential tool for researchers in life sciences, pharmaceutical development, and chemical biology who require high-fidelity probes for studying lipid metabolism, membrane dynamics, and intracellular trafficking.

Application

• Fluorescent Cell Imaging: Used as a vital stain for real-time visualization of cholesterol distribution and dynamics within live or fixed cells using fluorescence microscopy.
• Lipid Raft & Membrane Studies: Serves as a probe to investigate the organization, fluidity, and phase separation of lipid membranes and lipid raft domains.
• Intracellular Trafficking Assays: Tracks the uptake, transport, and metabolism of sterols in various cellular pathways, including endocytosis and exocytosis.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate compounds that modulate cholesterol homeostasis or related metabolic disorders.
• Biochemical Research: Acts as a substrate or tracer in enzymatic assays and in vitro studies related to sterol-modifying enzymes.
• Diagnostic Probe Development: Utilized in the research and development of novel diagnostic kits and assays for diseases associated with cholesterol dysregulation.

Basic Information

Product Name	23-(Dipyrrometheneboron Difluoride)-24-Norcholesterol
CAS No.	878557-19-8
Molecular Formula	C36H49BF2N2O
Molecular Weight	574.60 g/mol
Synonyms	BODIPY-cholesterol analog; BODIPY FL C12; 24-Nor-23-(4,4-difluoro-4-bora-3a,4a-diaza-s-indacen-8-yl)-5-cholen-3β-ol; BODIPY® FL C12; Fluorescent cholesterol probe; BODIPY-labeled cholesterol; BODIPY 500/510 C12; 23-(BODIPY)-24-norcholesterol
EINECS	Contact for details

Quality Control

Every batch of 23-(Dipyrrometheneboron Difluoride)-24-Norcholesterol is subjected to rigorous analytical testing to ensure the highest standards of purity and performance for research applications. Our quality protocols include comprehensive characterization to confirm structural identity and quantify fluorescent purity. A detailed Certificate of Analysis (COA) providing batch-specific data from HPLC, NMR, and mass spectrometry is available upon request to support your critical research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Fluorescence Properties	Ex/Em ~500/510 nm (in suitable solvent)
Solubility	Soluble in DMSO, DMF, ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.