Description

m-Peg3-Nhs Ester CAS NO 876746-59-7 is a heterobifunctional polyethylene glycol (PEG) linker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester at one terminus. This compound is essential for creating stable amide bonds between PEG spacers and primary amines in peptides, proteins, and other biomolecules, facilitating conjugation and bioconjugation strategies. It is a critical reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics industries who require precise and reproducible modification of biomolecules to enhance solubility, stability, and pharmacokinetic properties.

Application

• Bioconjugation and PEGylation: Covalent attachment of polyethylene glycol (PEG) chains to therapeutic peptides, proteins, and antibodies to improve solubility, reduce immunogenicity, and extend plasma half-life.
• Antibody-Drug Conjugate (ADC) Development: Serving as a key linker component for connecting cytotoxic payloads to monoclonal antibodies via amine groups.
• Diagnostic Reagent Synthesis: Conjugation of reporter molecules (e.g., fluorophores, enzymes) to antibodies, proteins, or oligonucleotides for use in immunoassays and biosensors.
• Biomaterial and Hydrogel Fabrication: Functionalizing surfaces or polymer backbones with PEG spacers to create biocompatible, non-fouling materials for medical devices and tissue engineering scaffolds.
• Crosslinking and Immobilization: Immobilizing enzymes or other proteins onto solid supports for biocatalysis, affinity chromatography, or microarray applications.
• Prodrug Synthesis: Designing prodrugs by linking active pharmaceutical ingredients (APIs) to promoieties via a cleavable or stable PEG linker.
• Nanoparticle Functionalization: Modifying the surface of liposomes, dendrimers, or polymeric nanoparticles with PEG chains to enhance stealth properties and targeting capabilities.
• Chemical Biology Probes: Creating tools for studying biological processes by linking affinity tags or imaging agents to small molecules or peptides.

Basic Information

Item	Details
Product Name	m-Peg3-Nhs Ester
CAS No.	876746-59-7
Molecular Formula	C15H23NO8
Molecular Weight	345.35 g/mol
Synonyms	m-PEG3-NHS ester; 3-((2-(2-(3-((2,5-Dioxopyrrolidin-1-yl)oxy)-3-oxopropoxy)ethoxy)ethoxy)propanoic acid NHS ester; α-Methyl-ω-(N-succinimidyloxycarbonyl) tri(ethylene glycol) propionate; NHS-PEG3-propionate; Propanoic acid, 3-[2-[2-(3-[(2,5-dioxo-1-pyrrolidinyl)oxy]-3-oxopropoxy)ethoxy]ethoxy]-, 2,5-dioxo-1-pyrrolidinyl ester; mPEG3-NHS Ester; Amine-reactive PEG linker; Heterobifunctional PEG NHS ester
EINECS	Contact for details

Quality Control

Our m-Peg3-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and MS for identity verification, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.