Description

Bisbenzimide H 33342 Trihydrochloride is a high-purity, cell-permeable fluorescent dye widely used as a vital nuclear stain in life science research. Its core value lies in its ability to bind preferentially to adenine-thymine (A-T) rich regions of DNA in live and fixed cells, providing a reliable and sensitive tool for chromatin visualization and cell cycle analysis. This compound is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and academic sectors, particularly for applications in fluorescence microscopy, flow cytometry, and high-content screening.

Application

• Live-Cell Nuclear Staining: For real-time visualization of nuclear morphology and chromatin dynamics in viable cells.
• Flow Cytometry Analysis: Used in DNA content and cell cycle analysis due to its stoichiometric DNA binding properties.
• Fluorescence Microscopy: A standard counterstain in multicolor fluorescent imaging to identify cell nuclei.
• High-Content Screening (HCS): Employed in automated imaging platforms for cell-based assays to quantify nuclear parameters.
• Apoptosis Detection: Helps in identifying characteristic nuclear changes like chromatin condensation and fragmentation.
• Microbiological Research: For staining DNA in bacteria, yeast, and other microorganisms.

Basic Information

Product Name	Bisbenzimide H 33342 Trihydrochloride
CAS No.	875756-97-1
Molecular Formula	C27H28N6O·3HCl
Molecular Weight	561.93 g/mol (Free base: 452.55)
Synonyms	Hoechst 33342 trihydrochloride; Bisbenzimide H 33342; HOE 33342; H33342; 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride; 2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-[2,5'-bi-1H-benzimidazole] trihydrochloride; Nuclear stain 33342; Cell-permeable DNA dye
EINECS	Contact for details

Quality Control

Our Bisbenzimide H 33342 Trihydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectral analysis for identity confirmation, and functional testing in cell-based assays. A detailed Certificate of Analysis (COA) documenting purity, absorbance/fluorescence profiles, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Absorbance (in water)	λmax ~343 nm
Fluorescence (in water, bound to DNA)	λex ~343 nm, λem ~483 nm
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Biological Activity (Cell Staining)	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.