Description

Cy3 Hydrazide is a reactive, near-infrared fluorescent dye derivative designed for the labeling and detection of biomolecules. This compound is essential for researchers and developers requiring high-sensitivity, stable conjugates for advanced bioanalytical applications. It is primarily used in the pharmaceutical, biotechnology, and diagnostic industries for applications such as fluorescence microscopy, flow cytometry, and in vivo imaging.

Application

• Bioconjugation and Probe Synthesis: Used to label antibodies, proteins, peptides, and oligonucleotides with the Cy3 fluorophore via hydrazone bond formation, specifically targeting aldehyde or ketone groups.
• Glycan and Carbohydrate Analysis: Employed in fluorescence-based assays for the detection and profiling of glycoproteins, polysaccharides, and other carbonyl-containing biomolecules.
• In Vivo and In Vitro Imaging: Serves as a key component in developing contrast agents and molecular probes for non-invasive fluorescence imaging in preclinical research.
• Diagnostic Assay Development: Integral to creating high-performance fluorescent lateral flow assays (LFAs), ELISA kits, and other immunoassay platforms.
• Flow Cytometry & Cell Sorting: Used to tag cell surface markers or intracellular targets for multiparametric analysis and fluorescence-activated cell sorting (FACS).
• High-Content Screening (HCS): Applied in automated microscopy platforms for drug discovery and cellular pathway analysis.

Basic Information

Product Name	Cy3 Hydrazide
CAS No.	871514-32-8
Molecular Formula	C34H41ClN4O2
Molecular Weight	573.18 g/mol
Synonyms	Cyanine3 hydrazide; Sulfo-Cy3 hydrazide (common variant); 2-((1E,3E)-5-(1-(6-Hydrazinyl-6-oxohexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3-ethyl-1,1-dimethyl-1H-benzo[e]indol-3-ium chloride; Cy3® Hydrazide; Cyanine 3 hydrazide; 1H-Benz[e]indolium, 2-[2-[2-[1-(6-hydrazinyl-6-oxohexyl)-3,3-dimethyl-1,3-dihydro-2H-indol-2-ylidene]ethylidene]-1-cyclopenten-1-yl]ethenyl]-3-ethyl-1,1-dimethyl-, chloride (1:1); Reactive Cy3 dye
EINECS	Contact for details

Quality Control

Our Cy3 Hydrazide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and spectrophotometry to verify optical properties. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.