Description

5(6)-FITC DA is a high-purity, fluorescein isothiocyanate (FITC) derivative specifically designed for advanced biochemical conjugation and labeling applications. This compound is critical for researchers and manufacturers requiring precise, fluorescent tagging of biomolecules for detection, quantification, and imaging. It is essential for professionals in the pharmaceutical, biotechnology, and diagnostic sectors developing assays, probes, and novel therapeutic conjugates.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to proteins, antibodies, peptides, and oligonucleotides for use in fluorescence microscopy, flow cytometry (FACS), and immunoassays (ELISA).
• Diagnostic Reagent Synthesis: A key intermediate in the production of fluorescent diagnostic kits and biosensors for clinical and research use.
• Cell Biology & Imaging: Preparation of fluorescent tracers for studying cell uptake, intracellular trafficking, and receptor binding studies.
• Bioconjugation Research: Used in method development for novel conjugation chemistries and linker technologies in drug discovery.
• Material Science: Functionalization of nanoparticles and polymers with fluorescent markers for tracking and biodistribution studies.
• High-Throughput Screening (HTS): Development of fluorescent probes for enzymatic activity assays and target identification in drug discovery pipelines.

Basic Information

Product Name	5(6)-FITC DA
CAS No.	871487-69-3
Molecular Formula	C26H17NO7S
Molecular Weight	463.48 g/mol
Synonyms	5(6)-Fluorescein Isothiocyanate-Diacetate; FITC-Diacetate; Fluorescein 5(6)-Isothiocyanate Diacetate; 5(6)-FITC Diacetate; 5- and 6-Isomers mixture of FITC-DA; 3',6'-Diacetylfluorescein Isothiocyanate; 2-(3',6'-Diacetyloxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl) Isothiocyanate
EINECS	Contact for details

Quality Control

Our 5(6)-FITC DA is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results for purity, absorbance, and fluorescence properties is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0% (sum of 5- and 6-isomers)
Absorbance (λmax)	~495 nm (in suitable solvent)
Emission (λmax)	~520 nm (in suitable solvent)
Solubility	Soluble in DMSO, DMF, acetone
Loss on Drying	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.