Description

5(6)-Fam-C6-Oh is a high-purity, fluorescent labeling reagent widely used in biochemical research and diagnostics. Its core value lies in its ability to act as a versatile linker, enabling the covalent attachment of fluorescent probes to biomolecules for detection and analysis. This product is essential for researchers and manufacturers in the fields of proteomics, nucleic acid sequencing, and diagnostic assay development who require reliable, high-quality labeling agents.

Application

• Fluorescent Labeling of Peptides and Proteins: Used as an amine-reactive succinimidyl ester (NHS ester) derivative for tagging biomolecules in fluorescence-based assays.
• Oligonucleotide Synthesis: Serves as a phosphoramidite precursor for incorporating fluorescent markers into DNA or RNA probes.
• Diagnostic Kit Manufacturing: A key component in the production of fluorescent conjugates for ELISA, lateral flow assays, and other in vitro diagnostic (IVD) devices.
• Cell Imaging and Microscopy: Used to prepare fluorescent tags for tracking cellular processes and localization studies.
• High-Throughput Screening (HTS): Enables the development of fluorescent tracers and sensors for drug discovery platforms.
• Bioconjugation and Cross-linking: Acts as a heterobifunctional linker for creating stable fluorescent antibody-drug conjugates (ADCs) or other bioconjugates.

Basic Information

Item	Detail
Product Name	5(6)-Fam-C6-Oh
CAS No.	869889-37-2
Molecular Formula	C31H29NO9
Molecular Weight	559.57 g/mol
Synonyms	5(6)-Carboxyfluorescein-C6-Alcohol; 6-(Fluorescein-5(6)-carboxamido)hexan-1-ol; FAM-C6-OH; 5(6)-FAM C6 Alcohol; 5/6-Carboxyfluorescein Hexanol; Fluorescein-C6-OH; 6-[(3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(or 6)-yl)carbonylamino]hexan-1-ol
EINECS	Contact for details

Quality Control

Our 5(6)-Fam-C6-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0% (Area %)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.