Description

Dnp-Peg4-Nhs Ester is a heterobifunctional crosslinking reagent featuring a dinitrophenyl (DNP) group and an N-hydroxysuccinimide (NHS) ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is essential for creating stable, site-specific conjugations in bioconjugation workflows, offering enhanced solubility and reduced aggregation compared to shorter linkers. It is primarily utilized by researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and advanced materials science for labeling proteins, antibodies, and other biomolecules.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via lysine residues.
• Diagnostic Probe Labeling: Preparation of enzyme- or fluorophore-labeled antibodies and antigens for ELISA, immunohistochemistry (IHC), and flow cytometry.
• Protein-Polymer Conjugation: Modification of therapeutic proteins with PEG chains to improve pharmacokinetic profiles (PEGylation).
• Surface Functionalization: Immobilization of biomolecules onto biosensor chips, microarrays, and nanoparticles for detection and capture applications.
• Affinity Chromatography Ligand Synthesis: Covalent attachment of proteins or peptides to solid supports for purification columns.
• Structural Biology Studies: Crosslinking agents for analyzing protein-protein interactions and complex structures.

Basic Information

Product Name	Dnp-Peg4-Nhs Ester
CAS No.	858126-78-0
Molecular Formula	C₂₅H₃₃N₃O₁₂
Molecular Weight	567.54 g/mol
Synonyms	NHS-PEG4-DNP; DNP-PEG4-NHS Ester; 2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2-(2,4-dinitrophenoxy)ethoxy)ethoxy)ethoxy)propanoate; Dinitrophenyl-PEG4-N-hydroxysuccinimide ester; N-(Dinitrophenyl)-triethyleneglycol-N-hydroxysuccinimide ester; DNP-PEG4-OSu; DNP tetraethylene glycol NHS ester
EINECS	Contact for details

Quality Control

Our Dnp-Peg4-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange solid or oil
Identification (HPLC/IR)	Conforms to structure
Purity (HPLC)	≥95%
Assay	≥90%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.