Description

Fluo-3 (Potassium Salt) is a high-purity, cell-permeant fluorescent calcium indicator widely used in life science research. This compound is essential for the quantitative measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells, providing critical insights into cellular signaling pathways. It is a vital tool for researchers and professionals in neurobiology, cardiology, pharmacology, and cell biology, enabling high-sensitivity detection of calcium flux in response to various stimuli.

Application

• Intracellular Calcium Imaging: Primary use for real-time visualization and quantification of cytosolic Ca²⁺ transients in live cells using fluorescence microscopy or flow cytometry.
• Neuroscience Research: Monitoring neuronal activity and synaptic transmission by detecting calcium spikes associated with action potentials and neurotransmitter release.
• Cardiovascular Studies: Investigating calcium signaling in cardiomyocytes to understand heart muscle contraction, arrhythmias, and drug effects.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) assays to identify compounds that modulate calcium channels or intracellular calcium release.
• Cell Physiology & Signal Transduction: Studying G-protein coupled receptor (GPCR) activation, ion channel function, and second messenger systems.
• Apoptosis & Cell Death Research: Detecting changes in intracellular calcium homeostasis that are early markers of programmed cell death.

Basic Information

Product Name	Fluo-3 (Potassium Salt)
CAS No.	853400-67-6
Molecular Formula	C₅₁H₄₈F₂K₂N₂O₂₃
Molecular Weight	1129.15 g/mol
Synonyms	Fluo-3 pentapotassium salt; Fluo-3, K⁺ salt; Fluo3 potassium salt; 2-[6-[Bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid pentapotassium salt; 2-[6-[Bis(2-carboxyethyl)amino]-5-[2-[2-[bis(2-carboxyethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]oxazole-5-carboxylic acid pentapotassium salt; Calcium indicator Fluo-3 K⁺ salt; BAPTA-based Ca²⁺ indicator
EINECS	Contact for details

Quality Control

Our Fluo-3 (Potassium Salt) is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot is characterized by multiple analytical techniques, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and the absence of interfering impurities. A comprehensive Certificate of Analysis (COA) detailing purity, fluorescence properties, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere or in sealed vials with desiccant. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Ex/Em ~506/526 nm (bound to Ca²⁺)
Solubility	Soluble in aqueous buffers, DMSO
Cell Permeability	Acetoxymethyl (AM) ester form available for loading

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.