Description

Atto 647 Acid is a high-performance, water-soluble fluorescent dye belonging to the cyanine dye family, identified by CAS NO 823803-95-8. This compound is prized for its exceptional photostability, high molar extinction coefficient, and bright far-red/near-infrared fluorescence emission, making it a superior choice for demanding detection applications. It is an essential tool for researchers and developers in the life sciences, diagnostics, and material science sectors who require reliable, high-sensitivity labeling.

Application

• Bioconjugation and Biomolecule Labeling: Covalently labeling proteins, antibodies, oligonucleotides, and peptides for fluorescence-based assays.
• Super-Resolution Microscopy (STORM, PALM): As a photoswitchable dye for imaging beyond the diffraction limit.
• Flow Cytometry and Cell Sorting: Used as a fluorescent tag for cell surface marker detection and intracellular staining.
• Fluorescence In Situ Hybridization (FISH): Providing high-contrast, photostable signals for genetic mapping and cytogenetics.
• Single-Molecule Detection: Enabling the tracking and visualization of individual molecules in complex biological systems.
• Diagnostic Assay Development: Serving as a critical component in the design of lateral flow tests, microarrays, and other diagnostic platforms.
• Materials Science and Nanotechnology: Functionalizing nanoparticles and polymers for advanced imaging and sensing applications.

Basic Information

Product Name	Atto 647 Acid
CAS No.	823803-95-8
Molecular Formula	C45H51N4O6S2+
Molecular Weight	~ 815.5 g/mol (as free acid)
Synonyms	ATTO 647 carboxylic acid; ATTO 647 COOH; 2-[7-[1,3-Dihydro-3,3-dimethyl-1-(4-sulfobutyl)-2H-indol-2-ylidene]-1,3,5-heptatrienyl]-3,3-dimethyl-1-(4-sulfobutyl)-3H-indolium-5-carboxylic acid; ATTO 647 SE precursor; Cyanine dye 647 acid; Far-red fluorescent dye acid; NIR dye acid 647
EINECS	Contact for details

Quality Control

Every batch of Atto 647 Acid is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and performance consistency. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes key parameters such as HPLC purity, absorbance/fluorescence profiles, and functional group verification. Our quality commitment supports applications in GMP-aligned diagnostic development and critical research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with molecular structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	644 ± 3 nm
Emission Maximum (λem)	669 ± 3 nm
Extinction Coefficient (ε)	≥ 120,000 L·mol⁻¹·cm⁻¹
Fluorescence Quantum Yield (φ)	Contact for details
Solubility	Soluble in water, DMSO, DMF
Functional Group (Carboxylic Acid)	Confirmed by titration or NMR

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.