Description

m-Tolyltetrazolium Red is a specialized tetrazolium salt derivative, primarily utilized as a vital biochemical reagent. Its core value proposition lies in its ability to serve as a sensitive redox indicator in cell viability and cytotoxicity assays, particularly for quantifying dehydrogenase enzyme activity. This compound is essential for research and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors. It enables precise measurement of metabolic activity in biological samples.

Application

• Cell Viability and Proliferation Assays: Used in MTT and related assays to measure mitochondrial activity in mammalian cell cultures.
• Microbiological Testing: Employed as an indicator for bacterial growth and metabolic activity in various microbiological studies.
• Enzyme Activity Studies: Acts as an electron acceptor in dehydrogenase enzyme assays, forming a colored formazan product.
• Toxicology Screening: Applied in in vitro toxicology to assess the cytotoxic effects of chemical compounds on cell lines.
• Pharmaceutical Research: Utilized in drug discovery and development for high-throughput screening of compound libraries.
• Plant Physiology Research: Used to study seed viability and root dehydrogenase activity.

Basic Information

Product Name	m-Tolyltetrazolium Red
CAS No.	88159-25-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	m-Tolyl Tetrazolium Red; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (common MTT salt structure variant); MTT Tetrazolium; Thiazolyl Blue Tetrazolium Bromide (related compound); Tetrazolium Red; TTC (Triphenyltetrazolium chloride) analogue; Cell Viability Assay Reagent; Dehydrogenase Indicator.
EINECS	Contact for details

Quality Control

Our m-Tolyltetrazolium Red is produced and tested under a strict quality management system to ensure batch-to-batch consistency and reliability for sensitive biochemical applications. Each lot undergoes rigorous analytical testing, including purity assessment by HPLC and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Solubility	Soluble in water and common organic solvents
Biological Activity	Conforms to reference standard in cell assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.