Description

Ethoxyfluorescein Ethyl Ester is a specialized fluorescent dye derivative, widely utilized as a vital intermediate and probe in advanced biochemical and pharmaceutical research. Its core value lies in its excellent fluorescence properties, which are essential for developing sensitive detection assays and diagnostic tools. This compound is particularly critical for researchers and manufacturers in the life sciences, analytical chemistry, and diagnostic reagent development sectors who require high-purity, reliable fluorescent markers.

Application

• Fluorescent Probe Synthesis: A key intermediate for creating custom fluorescent probes and tags used in cell biology and molecular imaging.
• Diagnostic Reagent Development: Used in the formulation of reagents for clinical diagnostics, including enzyme activity assays and immunoassays.
• Biochemical Research: Employed as a substrate or tracer in studying enzymatic processes, membrane permeability, and intracellular transport mechanisms.
• Analytical Chemistry Standards: Serves as a reference standard or calibrant in High-Performance Liquid Chromatography (HPLC) and fluorescence spectroscopy for method validation.
• Pharmaceutical Intermediate: Utilized in the research and development stages of novel therapeutic agents that incorporate fluorescent reporting groups.

Basic Information

Product Name	Ethoxyfluorescein Ethyl Ester
CAS No.	87569-96-8
Molecular Formula	C26H24O7
Molecular Weight	448.47 g/mol
Synonyms	5(6)-Carboxyfluorescein Diacetate N-Succinimidyl Ester (CFDA SE) analog; Ethoxyfluorescein Diacetate; 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein ethyl ester; Fluorescein O,O'-diacetic acid ethyl ester derivative; 3',6'-Bis(ethoxycarbonylmethyl)fluorescein ethyl ester; 5-Carboxyfluorescein diacetate ethyl ester; CellTrace™ CFSE related compound
EINECS	Contact for details

Quality Control

Our Ethoxyfluorescein Ethyl Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to guarantee traceability and compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Fluorescence Properties	Conforms to reference (Ex/Em ~492/517 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.