Description

Diamidino Yellow Dihydrochloride is a vital fluorescent nucleic acid stain used extensively in molecular biology and cytogenetics. Its core value lies in its high specificity for adenine-thymine (A-T) rich regions of DNA, enabling precise chromosome banding and nuclear staining. This compound is essential for researchers and professionals in the fields of life science research, clinical diagnostics, and pharmaceutical development who require reliable tools for chromosome analysis and cell biology studies.

Application

• Chromosome Banding (Q-banding): Primary application for producing characteristic fluorescent banding patterns on metaphase chromosomes for karyotyping and cytogenetic analysis.
• Nuclear and DNA Staining: Used in fluorescence microscopy to stain cell nuclei in fixed cells and tissue sections, aiding in the study of cell morphology and nuclear organization.
• Flow Cytometry: Employed as a DNA-selective fluorescent dye for cell cycle analysis and ploidy determination.
• Microbiological Research: Staining agent for detecting and visualizing specific bacteria and microorganisms based on their DNA content.
• Fluorescence In Situ Hybridization (FISH): Can be used as a counterstain in conjunction with specific probes to visualize chromosomal abnormalities and gene mapping.
• Apoptosis Detection: Useful in assays designed to identify apoptotic cells by changes in nuclear morphology and chromatin condensation.

Basic Information

Item	Detail
Product Name	Diamidino Yellow Dihydrochloride
CAS No.	87397-07-7
Molecular Formula	C₂₀H₂₂Cl₂N₈O
Molecular Weight	461.34 g/mol
Synonyms	Diamidino Yellow 2HCl; DAPI Yellow; Bisbenzimide Yellow; 2'-(4-Amidinophenyl)-6-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole Dihydrochloride; HOECHST S 769121; Yellow Fluorescent DNA Stain; A-T Selective DNA Stain; CAS 87397-07-7
EINECS	Contact for details

Quality Control

Our Diamidino Yellow Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for sensitive research applications. A Certificate of Analysis (COA) detailing identity, purity, and performance data is provided with every shipment to guarantee batch-to-batch consistency and reliability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorescence Performance	Conforms to reference standard
Solubility	Soluble in water and DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.