Description

4-(Methylmercapto)Phenyl Succinimidyl Succinate is a specialized heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a thiol-reactive methylmercapto group. This compound is critical for creating stable, site-specific conjugations between proteins, peptides, and other biomolecules, enabling advanced research and diagnostic applications. It is primarily utilized by scientists and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and the production of sensitive immunoassay reagents.

Application

• Bioconjugation and Protein Labeling: Site-specific coupling of peptides, antibodies, or enzymes with reporter molecules (e.g., fluorophores, enzymes, drugs).
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serves as a key chemical building block for constructing cleavable or non-cleavable linkers in targeted cancer therapeutics.
• Immunoassay Reagent Development: Used to prepare enzyme-labeled antibodies or antigens for ELISA and other diagnostic test kits.
• Surface Modification and Immobilization: Functionalization of solid supports (e.g., beads, chips, plates) with biomolecules for biosensors and affinity purification.
• Polymer and Biomaterial Science: Crosslinking agent for modifying hydrogels or polymers to introduce bioactive thiol or amine-reactive sites.

Basic Information

Product Name	4-(Methylmercapto)Phenyl Succinimidyl Succinate
CAS No.	86451-38-9
Molecular Formula	C15H15NO6S
Molecular Weight	337.35 g/mol
Synonyms	4-(Methylthio)phenyl succinimidyl succinate; SM(PEG)4 (commonly used trade name for the PEGylated analog family); 4-(Methylsulfanyl)phenyl 2,5-dioxopyrrolidin-1-yl butanedioate; Succinimidyl 4-(methylthio)phenyl succinate; NHS-PEG4-Maleimide precursor (related compound); Heterobifunctional crosslinker, NHS-SS-Phenyl-SCH3; 4-MTPS
EINECS	Contact for details

Quality Control

Our 4-(Methylmercapto)Phenyl Succinimidyl Succinate is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for research and bioconjugation applications. Each lot is characterized by NMR, HPLC, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.