Description

Sulforhodamine B 5-Acid Fluoride is a highly reactive, fluorescent labeling reagent derived from the sulforhodamine B dye scaffold. This compound is specifically engineered for the covalent attachment of fluorescent tags to biomolecules, offering superior stability and brightness. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, diagnostic assay development, and advanced fluorescence microscopy, where reliable and intense signal generation is critical.

Application

• Fluorescent Labeling of Proteins and Antibodies: Used to create bright, photostable conjugates for immunoassays (ELISA, flow cytometry) and in vivo imaging.
• Diagnostic Probe Synthesis: A key intermediate in manufacturing fluorescent probes and kits for clinical diagnostics and point-of-care testing.
• Biomolecule Tracking: Enables long-term, high-sensitivity tracking of cellular uptake, receptor binding, and intracellular trafficking.
• Polymer and Material Science: Functionalization of nanoparticles, polymers, and surfaces for sensor development and advanced material characterization.
• Pharmaceutical Research: Labeling of drug candidates and delivery vehicles for pharmacokinetic and biodistribution studies.
• Nucleic Acid Labeling: Conjugation to oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.

Basic Information

Product Name	Sulforhodamine B 5-Acid Fluoride
CAS No.	86368-10-7
Molecular Formula	C27H20F3N2O7S2
Molecular Weight	605.58 g/mol
Synonyms	Sulforhodamine 101 acid fluoride; SRB 5-Acid Fluoride; Texas Red-X acid fluoride; 5-(and-6)-Carboxy-X-rhodamine 5(6)-acid fluoride; Lissamine rhodamine B sulfonyl fluoride; Sulforhodamine B sulfonyl fluoride; 3',6'-Bis(diethylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-5'(6')-carboxylic acid, 2-sulfo-, 5(6)-fluoride
EINECS	Contact for details

Quality Control

Our Sulforhodamine B 5-Acid Fluoride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional testing for labeling efficiency. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Activity (Labeling)	≥ 90% coupling efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.