Description

Neurodye Rh-237 is a high-purity, lipophilic membrane potential-sensitive fluorescent dye widely used in neuroscience and cellular biophysics research. Its core value lies in its ability to provide fast, sensitive, and reliable optical measurements of transmembrane potential changes in excitable cells, such as neurons and cardiomyocytes. This compound is essential for researchers and manufacturers in the fields of neurobiology, cardiac electrophysiology, and high-throughput screening for drug discovery.

Application

• Neuroscience Research: Optical monitoring of action potentials and membrane potential dynamics in neuronal cultures, brain slices, and in vivo models.
• Cardiac Electrophysiology: High-speed imaging of action potentials and arrhythmia studies in isolated cardiomyocytes and cardiac tissue.
• Drug Discovery & Safety Pharmacology: Screening for compounds that modulate ion channel activity and assessing cardiotoxicity in high-throughput assay systems.
• Cellular Biophysics: Investigating mitochondrial membrane potential and energy metabolism in various cell types.
• Fluorescent Probe & Sensor Development: Serving as a key component in the formulation of advanced optical sensors and imaging kits.
• In Vitro Diagnostic (IVD) Reagents: Used in the development of research-use-only (RUO) assay kits for cellular function analysis.

Basic Information

Product Name	Neurodye Rh-237
CAS No.	83668-91-1
Molecular Formula	C29H31N2O3
Molecular Weight	455.57 g/mol
Synonyms	RH 237; RH237; Neuro-Dye RH-237; Di-4-ANEPPS; 4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)pyridinium inner salt; JPW 1114; Voltage-sensitive dye RH237; Potential-sensitive dye 237; Di-4-ANEPPS (RH237); 1-(3-Sulfopropyl)-4-[β-[2-(dibutylamino)-6-naphthyl]vinyl]pyridinium betaine
EINECS	Contact for details

Quality Control

Our Neurodye Rh-237 is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence profiles, and performance data is provided with every shipment to support your research and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under subdued lighting conditions to maintain stability.

Specification

Item	Specification
Appearance	Dark green to purple solid or powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Absorbance (λmax in Methanol)	~ 530 nm
Emission (λmax in Methanol)	~ 715 nm
Solubility	Soluble in DMSO, DMF, ethanol; sparingly soluble in aqueous buffers
Loss on Drying	≤ 1.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.