Description

Chloromethylketone Methotrexate is a key chemical intermediate, specifically a chloromethyl ketone derivative of the antifolate drug methotrexate. This compound is of significant value in pharmaceutical research and development for the synthesis of novel drug candidates and targeted conjugates. It is primarily utilized by research institutions, pharmaceutical companies, and fine chemical manufacturers engaged in oncology drug discovery and the development of antibody-drug conjugates (ADCs).

Application

• Pharmaceutical Intermediate: A critical building block for the synthesis of novel methotrexate analogs and prodrugs with potential therapeutic applications.
• Antibody-Drug Conjugate (ADC) Payload Synthesis: Serves as a precursor for constructing potent cytotoxin payloads designed for targeted cancer therapies.
• Biochemical Research: Used in studies investigating enzyme inhibition, particularly targeting dihydrofolate reductase (DHFR), and for probing folate metabolism pathways.
• Medicinal Chemistry: Employed in structure-activity relationship (SAR) studies to develop new compounds with optimized efficacy and reduced toxicity profiles.
• High-Purity Reference Standard: Supplied as a characterized compound for analytical method development and quality control in pharmaceutical manufacturing.

Basic Information

Product Name	Chloromethylketone Methotrexate
CAS No.	83160-47-8
Molecular Formula	C21H22ClN8O5
Molecular Weight	501.90 g/mol
Synonyms	4-Amino-10-methylpteroyl-γ-chloromethylglutamic acid; N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-γ-chloromethyl-L-glutamic acid; Methotrexate chloromethyl ketone; MTX chloromethyl ketone; L-γ-Chloromethylglutamic acid N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-; Chloromethyl ketone derivative of Methotrexate
EINECS	Contact for details

Quality Control

Our Chloromethylketone Methotrexate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods (NMR, MS). We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. The material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to light brown solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.