Description

Acetamidofluorescein is a chemically modified derivative of fluorescein, designed for advanced biochemical and analytical applications. This compound is valued for its enhanced photostability and specific reactivity, making it a superior choice for creating highly sensitive and stable fluorescent probes. It is primarily utilized by researchers and manufacturers in the fields of life sciences, diagnostics, and chemical biology for labeling biomolecules and developing assay systems.

Application

• Fluorescent Labeling Agent: Used for covalent conjugation to proteins, antibodies, and nucleic acids to create detection probes for immunoassays (e.g., ELISA, Western blot) and fluorescence in situ hybridization (FISH).
• Biochemical Research: Serves as a critical reagent in enzyme activity assays and receptor-ligand binding studies due to its bright fluorescence and specific chemical handle.
• Diagnostic Kit Development: Incorporated into lateral flow assays and other point-of-care diagnostic devices as a signal-generating component.
• Cell Biology & Imaging: Employed for labeling cellular components and tracking biological processes in live-cell imaging and flow cytometry.
• Chemical Biology Probes: Acts as a building block for synthesizing activity-based probes to study enzyme function and metabolic pathways.
• Material Science: Used in the development of fluorescent sensors and smart materials for environmental or chemical detection.

Basic Information

Product Name	Acetamidofluorescein
CAS No.	82779-14-4
Molecular Formula	C24H17NO7
Molecular Weight	431.40 g/mol
Synonyms	5-(Acetylamino)fluorescein; 5-Acetamidofluorescein; Fluorescein 5-acetamide; N-Acetyl-5-aminofluorescein; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-(acetylamino)benzoic acid; 5'-Aminofluorescein N-acetate; Acetamido FITC precursor
EINECS	Contact for details

Quality Control

Our Acetamidofluorescein is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification (IR, NMR), and residual solvent analysis, to ensure it meets the high standards required for research and diagnostic applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Fluorescence Properties	Ex/Em maxima consistent with standard
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.