Description

Tetramethylrhodamine Iodoacetamide is a high-purity, reactive fluorescent dye derivative used for site-specific labeling of thiol groups in proteins and peptides. This compound is critical for researchers and manufacturers requiring precise, stable fluorescent conjugates for advanced analytical and diagnostic applications. It is primarily utilized in the life sciences sector for proteomics research, fluorescence microscopy, flow cytometry, and the development of bioassays and diagnostic kits.

Application

• Protein & Peptide Labeling: Covalent tagging of cysteine residues for fluorescence-based studies of protein structure, function, and interactions.
• Fluorescence Microscopy: Preparation of fluorescent probes for cellular and subcellular imaging with high photostability and red emission.
• Flow Cytometry: Conjugation to antibodies or other biomolecules for cell surface marker detection and analysis.
• Bioconjugation & Probe Development: Synthesis of custom fluorescent probes and reagents for diagnostic assay development.
• Gel Electrophoresis & Western Blotting: Pre-staining of protein samples for in-gel detection and analysis.
• FRET (Förster Resonance Energy Transfer) Studies: Acting as an acceptor or donor dye in molecular proximity assays.

Basic Information

Product Name	Tetramethylrhodamine Iodoacetamide
CAS No.	81235-33-8
Molecular Formula	C25H22IN3O4
Molecular Weight	555.37 g/mol
Synonyms	5-Iodoacetamido-tetramethylrhodamine; TMRIA; Tetramethylrhodamine-5-iodoacetamide; 5-TMRIA; 5-(Iodoacetamido)tetramethylrhodamine; Rhodamine-based iodoacetamide; TAMRA iodoacetamide; Fluorescent thiol-reactive probe
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine Iodoacetamide is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and reactivity. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Extinction Coefficient	~95,000 cm-1M-1 at 555 nm
Emission Maximum	~580 nm
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.