Description

Methyl Calcein Blue is a high-purity, fluorescent chelating agent widely used as a sensitive indicator for calcium ions. Its core value lies in its high selectivity and strong fluorescence enhancement upon binding with calcium, making it superior for trace analysis. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and analytical chemistry sectors who require precise metal ion detection.

Application

• Fluorometric Calcium Assays: Primary application as a selective fluorescent probe for the quantitative determination of free calcium ions (Ca²⁺) in biochemical and clinical research.
• Cell Biology & Physiology Studies: Used in fluorescence microscopy and flow cytometry to monitor intracellular calcium flux and signaling in live cells.
• Analytical Chemistry Reagent: Employed in the development of sensitive optical sensors and test kits for calcium detection in various matrices.
• Quality Control in Pharmaceuticals: Acts as a key component in assays to monitor calcium levels in drug formulations and raw materials.
• Environmental & Industrial Analysis: Applied for trace calcium measurement in water quality testing and process streams.
• Research & Development: Serves as a fundamental tool in metalloenzyme studies and the development of new chelation-based diagnostic methods.

Basic Information

Product Name	Methyl Calcein Blue
CAS No.	81028-96-8
Molecular Formula	C₃₁H₂₈N₂O₁₃
Molecular Weight	636.56 g/mol
Synonyms	MCB; Methylcalcein Blue; 3,3'-Bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonphthalein; o-Cresolsulfonphthalein-3,3'-bis(methyliminodiacetic acid); Fluorescent Calcium Indicator MCB; Calcium Green; Calcein Blue derivative
EINECS	Contact for details

Quality Control

Our Methyl Calcein Blue is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectroscopic identification, and heavy metal screening, to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, performance, and trace impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture, which can affect performance.

Specification

Item	Specification
Appearance	Orange to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence Properties	Ex/Em maxima conforms
Calcium Binding Assay	Performance meets reference standard
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.