Description

6-Aminotetramethylrhodamine is a high-purity, amine-reactive derivative of the classic rhodamine dye family, widely recognized for its bright orange-red fluorescence. This compound is a critical building block for creating fluorescent probes and conjugates with exceptional photostability and high quantum yield. It is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require reliable, high-performance labeling reagents for bioimaging, flow cytometry, and nucleic acid detection.

Application

• Fluorescent Labeling Reagent: Primary use as an amine-reactive succinimidyl ester (6-TAMRA, SE) for covalently labeling proteins, peptides, antibodies, and oligonucleotides.
• Bioconjugation & Probe Synthesis: A key intermediate in the synthesis of custom fluorescent probes, molecular beacons, and FRET (Förster Resonance Energy Transfer) pairs.
• Life Science Research: Indispensable in fluorescence microscopy, confocal imaging, and intracellular tracking studies due to its excellent spectral properties.
• Diagnostic Assays: Used in the development of fluorescent immunoassays (FLISA) and in situ hybridization (FISH) kits for clinical and research diagnostics.
• Flow Cytometry: Employed as a fluorescent tag for cell surface marker staining and intracellular protein detection in multiparameter flow analysis.
• Nucleic Acid Analysis: Labeling of primers, probes, and dUTP for PCR, sequencing, and microarray applications.
• Material Science: Potential use in the development of fluorescent sensors and functional materials.

Basic Information

Product Name	6-Aminotetramethylrhodamine
CAS No.	80724-18-1
Molecular Formula	C25H23N3O3
Molecular Weight	413.47 g/mol
Synonyms	6-TAMRA; 6-Carboxytetramethylrhodamine amine; 5(6)-TAMRA; 6-Amino-3',6'-bis(dimethylamino)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Rhodamine 6G amine; 6-TAMRA amine; 6-Amino-TMR; 6-ATR
EINECS	Contact for details

Quality Control

Our 6-Aminotetramethylrhodamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry for fluorescence properties. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all specifications to ensure traceability and compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and light-sensitive; desiccate under inert atmosphere for long-term storage. Keep away from strong acids and oxidizing agents.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Absorption Maximum (λmax)	~550 nm
Emission Maximum (λem)	~575 nm
Water Content (KF)	≤ 2.0%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.