Description

2',7'-Dichlorofluorescein Sodium Salt is a high-purity, water-soluble derivative of fluorescein, widely recognized as a critical fluorescent probe and indicator dye. Its primary value lies in its exceptional sensitivity for detecting reactive oxygen species (ROS) and peroxides, making it indispensable for biochemical research and analytical assays. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic industries, where precise oxidative stress measurement is required.

Application

• Oxidative Stress Detection: As the substrate for the DCFH-DA assay, it is the gold standard for measuring intracellular levels of hydrogen peroxide and reactive oxygen species (ROS) in cell biology and toxicology studies.
• Biochemical Research Reagent: Used as a sensitive fluorescent probe in enzymatic assays and studies of antioxidant activity and free radical chemistry.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to evaluate the efficacy of novel antioxidant and cytoprotective drug candidates.
• Diagnostic Kit Manufacturing: A key component in the formulation of commercial diagnostic kits for oxidative stress markers and related pathological conditions.
• Analytical Chemistry: Functions as a fluorescent indicator and tracer in various chromatographic and capillary electrophoresis techniques.
• Material Science: Can be utilized in the development of sensor materials and films for detecting environmental oxidants.

Basic Information

Product Name	2',7'-Dichlorofluorescein Sodium Salt
CAS No.	80471-69-8
Molecular Formula	C20H9Cl2NaO5
Molecular Weight	423.18 g/mol
Synonyms	DCFH Sodium Salt; 2',7'-Dichlorofluorescein, Sodium Salt; DCF Sodium Salt; 2',7'-Dichlorodihydrofluorescein Sodium Salt (precursor form); DCFH-DA Hydrolysis Product; Sodium 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthene]-3-one; Reactive Oxygen Species (ROS) Indicator; Fluorescein, 2',7'-dichloro-, sodium salt
EINECS	Contact for details

Quality Control

Our 2',7'-Dichlorofluorescein Sodium Salt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometric analysis for correct absorbance/fluorescence profiles, and residual solvent testing. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Fluorescence Properties	Conforms to reference
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.