Description

Calcofluor White Rwp is a high-purity, fluorescent brightener and optical brightening agent (OBA) widely used as a vital diagnostic stain. Its core value lies in its exceptional affinity for binding to polysaccharides like cellulose and chitin, producing a strong, stable blue-white fluorescence under ultraviolet light. This property makes it indispensable for researchers and quality control professionals in life sciences, pulp and paper analysis, and textile manufacturing who require precise detection and visualization of specific biological structures and materials.

Application

• Microscopy & Cell Biology: A critical stain for visualizing fungal elements (e.g., Pneumocystis jirovecii), plant cell walls, and other β-glucan structures in clinical and research laboratories.
• Pulp & Paper Industry: Used in quality control to assess fiber composition, measure cellulose content, and detect imperfections or contaminants in paper products.
• Textile Manufacturing: Employed to evaluate the effectiveness of finishing processes and to study fiber morphology and polymer distribution.
• Biofilm Research: Stains the polysaccharide matrix of microbial biofilms, enabling detailed study of their structure and development.
• Plant Pathology & Mycology: Facilitates the rapid identification and study of plant pathogens, especially fungi, by staining their cell walls.
• Material Science: Used in the analysis of composite materials and natural polymers to study binding sites and material integrity.

Basic Information

Product Name	Calcofluor White Rwp
CAS No.	8066-05-5
Molecular Formula	C40H44N12O10S2 · 2Na
Molecular Weight	960.97 g/mol (as disodium salt)
Synonyms	Calcofluor White M2R; Fluorescent Brightener 28; Tinopal UNPA-GX; Cellufluor; FWA 140; Blankophor RWP; 4,4'-Bis[[4-[bis(2-hydroxyethyl)amino]-6-(4-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulfonic acid disodium salt; Direct White RWP
EINECS	Contact for details

Quality Control

Our Calcofluor White Rwp is manufactured under strict quality management systems to ensure batch-to-batch consistency and performance reliability for sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for fluorescence intensity verification, and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive; prolonged exposure to light may degrade its fluorescent properties. Keep the container tightly sealed when not in use to prevent moisture absorption.

Specification

Item	Specification
Appearance	Off-white to pale yellow powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Fluorescence Intensity	≥ 95% of reference standard
Loss on Drying	≤ 5.0%
Solubility (in Water)	Clear, slightly opalescent solution
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.