Description

4-Nitrophenyl Acetate is a high-purity organic ester compound widely utilized as a critical reagent in biochemical and pharmaceutical research. Its primary value lies in its role as a chromogenic substrate for the detection and kinetic analysis of esterase and lipase enzyme activity. This compound is essential for research laboratories, diagnostic kit manufacturers, and pharmaceutical development teams working on enzyme inhibition studies, high-throughput screening, and biochemical assays.

Application

• Enzyme Substrate: Acts as a standard chromogenic substrate for spectrophotometric assays of esterases, lipases, and acetylcholine esterase.
• Biochemical Research: Used in kinetic studies to determine enzyme activity, inhibition constants (Ki), and catalytic mechanisms.
• Diagnostic Reagent: A key component in the formulation of clinical and research diagnostic kits for liver function and other enzyme-linked tests.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential therapeutic enzyme inhibitors.
• Chemical Synthesis: Serves as a protected intermediate or acylating agent in organic synthesis and peptide chemistry.
• Quality Control: Used as a reference standard in the quality control of enzyme preparations and diagnostic products.

Basic Information

Product Name	4-Nitrophenyl Acetate
CAS No.	830-03-5
Molecular Formula	C8H7NO4
Molecular Weight	181.15 g/mol
Synonyms	p-Nitrophenyl acetate; 4-Nitrophenyl ethanoate; PNPA; PNP-acetate; 4-Acetoxynitrobenzene; 4-Nitrophenol acetate; Acetic acid 4-nitrophenyl ester; 1-Acetoxy-4-nitrobenzene
EINECS	212-598-9

Quality Control

Our 4-Nitrophenyl Acetate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, IR and NMR for identification, and loss on drying to ensure it meets the stringent requirements for research and diagnostic applications. A detailed Certificate of Analysis (COA) with lot-specific data is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at a controlled room temperature (15-25°C). Keep away from heat, open flames, and incompatible materials such as strong bases and oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	77 - 81 °C
Loss on Drying	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.