Description

Methyl Green is a synthetic cationic dye belonging to the triarylmethane class, widely recognized for its distinctive green color and specific binding affinity for nucleic acids. This compound is a critical reagent in life science research, particularly for histological staining and cytological analysis. It is essential for laboratories and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors requiring high-purity biological stains for accurate visualization and quantification.

Application

• Histological Staining: Used as a component in staining solutions, such as the Methyl Green-Pyronin stain, to differentiate DNA (stains green) from RNA (stains red) in tissue sections.
• Cytology and Microscopy: Employed for staining cell nuclei in smears and fixed preparations, aiding in the study of cell morphology and nuclear details.
• Gel Staining: Can be used as an alternative stain for visualizing nucleic acids in agarose or polyacrylamide gels after electrophoresis.
• Biochemical Research: Utilized in various assays to study nucleic acid-protein interactions and chromatin structure due to its selective binding properties.
• Educational Kits: A key component in ready-to-use staining kits for academic and training laboratories in biology and medicine.
• Industrial Dye: Finds application as a colorant in specific non-biological contexts, such as inks and pigments, where its stable green hue is required.

Basic Information

Product Name	Methyl Green
CAS No.	82-94-0
Molecular Formula	C26H33Cl2N3
Molecular Weight	458.47 g/mol
Synonyms	Basic Green 5; C.I. 42585; Ethyl Green; Methyl Green O; Methylgreen; Methyl Green Zinc Chloride Salt; [4-[[4-(Dimethylamino)phenyl][4-(methylamino)-1-naphthalenyl]methylene]-2,5-cyclohexadien-1-ylidene]dimethylammonium chloride zinc chloride salt
EINECS	201-455-1

Quality Control

Our Methyl Green is produced and tested to meet the stringent requirements of research and industrial applications. Each batch undergoes rigorous analysis to ensure consistent purity, color strength, and performance in staining protocols. A comprehensive Certificate of Analysis (COA) detailing identity, purity (assay), and impurity profiles is provided with every shipment to guarantee traceability and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Keep away from incompatible materials. The product is light-sensitive and should be stored away from direct light exposure to maintain stability.

Specification

Item	Specification
Appearance	Dark green powder or crystals with metallic luster
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 85% (as Methyl Green)
Loss on Drying	≤ 5.0 %
Solubility	Soluble in water, slightly soluble in ethanol
Absorbance (λ max)	630 - 635 nm (in water)
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.