Description

5-Fam Maleimide is a high-purity, fluorescent labeling reagent designed for the site-specific conjugation of biomolecules. This compound is critical for researchers and manufacturers requiring precise, stable fluorescent tagging for analytical and diagnostic applications. It is primarily utilized in the life sciences, pharmaceutical development, and biotechnology sectors for applications such as protein labeling, antibody-drug conjugate (ADC) development, and fluorescence-based assays.

Application

• Protein and Antibody Labeling: Site-specific conjugation of fluorescent tags to cysteine residues for imaging, flow cytometry, and Western blotting.
• Antibody-Drug Conjugate (ADC) Development: Used as a linker-payload component for creating fluorescently labeled ADCs for research and tracking.
• Bioconjugation & Probe Synthesis: Essential for synthesizing fluorescent probes and sensors for molecular detection and cellular imaging.
• Diagnostic Assay Development: Key reagent in the manufacture of fluorescent immunoassays and other diagnostic kits.
• Biomolecular Interaction Studies: Enables fluorescence resonance energy transfer (FRET) and other proximity-based assays to study protein-protein interactions.
• Polymer & Material Science: Functionalization of polymers and surfaces with fluorescent markers for advanced material characterization.

Basic Information

Product Name	5-Fam Maleimide
CAS No.	787632-00-2
Molecular Formula	C24H15NO7
Molecular Weight	429.38 g/mol
Synonyms	5-Carboxyfluorescein Maleimide; 5-FAM Maleimide; 5-FAM-C5-Maleimide; FAM Maleimide; Fluorescein-5-Maleimide; 6-Fluorescein-5(6)-carboxamidohexanoic acid, N-(2,5-Dioxo-1-pyrrolidinyl) ester
EINECS	Contact for details

Quality Control

Our 5-Fam Maleimide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation, to ensure it meets the high standards required for sensitive bioconjugation work. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccated conditions. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Absorption (λmax)	~492 nm
Emission (λmax)	~518 nm
Solubility	DMSO, DMF
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.