Description

n-(5-Aminopentyl)-N'-(3',6'-Dihydroxy-3-Oxospiro[Isobenzofuran-1(3H),9'-[9H]Xanthen]-5-Yl)Thiourea Dihydrobromide is a high-purity, functionalized chemical derivative of fluorescein, designed for advanced research and diagnostic applications. This compound is critical for scientists and manufacturers requiring a reliable, well-characterized building block for creating sensitive detection probes. It is primarily utilized in the development of novel fluorescent labels, biosensors, and assay reagents for the life sciences and pharmaceutical industries.

Application

• Fluorescent Probe Synthesis: Acts as a key intermediate for creating custom fluorescent dyes and labels with reactive amine handles for conjugation to biomolecules.
• Bioconjugation & Assay Development: Used in the preparation of fluorescently tagged antibodies, peptides, and nucleic acids for immunoassays, flow cytometry, and in-situ hybridization.
• Biosensor Fabrication: Serves as a critical component in the design of optical chemical sensors and bioimaging agents due to its derivatizable structure and fluorescent core.
• Pharmaceutical Research: Employed in drug discovery for labeling target molecules to study binding interactions, cellular uptake, and pharmacokinetics.
• Diagnostic Reagent Manufacturing: Integrated into the production of high-sensitivity diagnostic kits for clinical and research laboratories.
• Material Science Research: Used in the development of smart materials and functional surfaces where controlled fluorescence properties are required.

Basic Information

Product Name	n-(5-Aminopentyl)-N'-(3',6'-Dihydroxy-3-Oxospiro[Isobenzofuran-1(3H),9'-[9H]Xanthen]-5-Yl)Thiourea Dihydrobromide
CAS No.	786705-84-8
Molecular Formula	C28H28Br2N4O5S
Molecular Weight	684.41 g/mol
Synonyms	5-(5-Aminopentyl)thioureidofluorescein Dihydrobromide; Fluorescein-5-isothiocyanate (5-FITC) Aminopentyl Derivative Dihydrobromide; 5-FITC-C5-NH2 Dihydrobromide; 5-(5-Aminopentylcarbamothioylamino)fluorescein Dihydrobromide; Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-[[(5-aminopentyl)amino]thioxomethyl]amino]-3',6'-dihydroxy-, Dihydrobromide
EINECS	Contact for details

Quality Control

Our production of n-(5-Aminopentyl)-N'-(3',6'-Dihydroxy-3-Oxospiro[Isobenzofuran-1(3H),9'-[9H]Xanthen]-5-Yl)Thiourea Dihydrobromide adheres to stringent quality management protocols. Each batch is subjected to comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and rigorous impurity profiling. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with research-grade material standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.