Description

Cyanine 3 Bihexanoic Acid Dye, Potassium Salt is a reactive, water-soluble near-infrared fluorescent dye belonging to the cyanine dye family. This compound is engineered for superior stability and conjugation efficiency, making it an essential tool for advanced bioimaging and diagnostic applications. It is primarily used by researchers and manufacturers in the biotechnology, pharmaceutical, and in vitro diagnostics (IVD) sectors for labeling biomolecules such as proteins, peptides, and oligonucleotides.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and nucleic acids (DNA/RNA) for detection and quantification.
• Bioimaging and Microscopy: Used as a probe for fluorescence microscopy (including confocal and super-resolution), flow cytometry, and in vivo imaging in the Cy3 channel.
• Diagnostic Assay Development: A key component in the manufacture of fluorescent lateral flow assays (LFAs), ELISA kits, and other immunoassay platforms.
• Nucleic Acid Detection: Labeling of probes for fluorescence in situ hybridization (FISH), PCR, and microarray analysis.
• Drug Discovery & High-Throughput Screening (HTS): Tagging of targets or compounds in cell-based assays and binding studies.
• Material Science & Sensor Development: Incorporation into polymers or nanoparticles to create fluorescent sensors and functional materials.

Basic Information

Product Name	Cyanine 3 Bihexanoic Acid Dye, Potassium Salt
CAS No.	762260-71-9
Molecular Formula	C37H45KN2O6S2
Molecular Weight	716.99 g/mol
Synonyms	Cy3 Bihexanoic Acid Potassium Salt; Sulfo-Cyanine3 acid, Potassium Salt; Cy3™ carboxylic acid derivative; 1-(6-Carboxyhexyl)-2-[(1E,3E)-3-(1-{6-[carboxylato(oxido)amino]-6-oxohexyl}-3,3-dimethyl-5-sulfo-1,3-dihydro-2H-indol-2-ylidene)prop-1-en-1-yl]-3,3-dimethyl-3H-indol-1-ium-5-sulfonate, Potassium Salt; Potassium 2-((1E,3E)-3-(1-(6-carboxyhexyl)-3,3-dimethyl-5-sulfo-2,3-dihydro-1H-indol-2-ylidene)prop-1-en-1-yl)-1-(6-carboxyhexyl)-3,3-dimethyl-5-sulfo-3H-indol-1-ium-1-ide; Cy3 NHS ester precursor; Reactive Cy3 dye acid salt
EINECS	Contact for details

Quality Control

Our Cyanine 3 Bihexanoic Acid Dye, Potassium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry for absorbance and fluorescence verification, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark green to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.