Description

Atto 565 is a bright, photostable fluorescent dye belonging to the cyanine dye family, widely recognized for its high molar extinction coefficient and excellent quantum yield. Its primary value lies in providing superior signal-to-noise ratios in demanding fluorescence-based detection and imaging applications, making it a critical reagent for advanced research and diagnostics. This dye is essential for scientists and manufacturers in the fields of life sciences, molecular diagnostics, and flow cytometry who require reliable and intense labeling for biomolecules.

Application

• Fluorescence Labeling of Biomolecules: Conjugation to antibodies, oligonucleotides, peptides, and proteins for use in immunoassays (e.g., ELISA, Western Blot) and nucleic acid detection.
• Flow Cytometry & Cell Sorting (FACS): Used as a fluorescent tag for cell surface markers and intracellular targets, enabling high-resolution cell population analysis and isolation.
• Fluorescence In Situ Hybridization (FISH): Labeling of DNA probes for chromosomal analysis, genetic testing, and microbiological identification.
• Microscopy & Bioimaging: Application in confocal, super-resolution, and live-cell imaging due to its brightness and photostability.
• Diagnostic Assay Development: Integration into lateral flow assays, microarrays, and other point-of-care diagnostic platforms.
• Polymerase Chain Reaction (PCR) Probes: Serving as a donor or acceptor dye in FRET-based qPCR and real-time PCR applications.

Basic Information

Product Name	Atto 565
CAS No.	722538-90-1
Molecular Formula	C37H47N4O7S2+ • Anion-
Molecular Weight	~723.92 g/mol (cation)
Synonyms	ATTO 565; ATTO-565; 2-[5-[1,3-Dihydro-3,3-dimethyl-1-(4-sulfobutyl)-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-3,3-dimethyl-1-(4-sulfobutyl)-3H-indolium; ATTO 565 dye; C37H47N4O7S2; Sulfoindocyanine dye 565
EINECS	Contact for details

Quality Control

Every batch of Atto 565 is manufactured under a strict quality management system and undergoes comprehensive analytical testing. We ensure high purity and consistent performance through methods including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, absorbance/fluorescence profiles, and functional performance are provided with each shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to prevent condensation. The material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark green to purple solid or solution
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	563 ± 3 nm
Emission Maximum (λem)	590 ± 5 nm
Extinction Coefficient (ε)	≈ 120,000 L·mol⁻¹·cm⁻¹
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.