Description

5(6)-Nitrofluorescein is a nitro-substituted derivative of fluorescein, a key synthetic intermediate and analytical reagent. This compound is valued for its role in the development of fluorescent probes, dyes, and biological staining agents. It is primarily utilized by research institutions, diagnostic kit manufacturers, and fine chemical producers in the life sciences and analytical chemistry sectors.

Application

• Fluorescent Probe Synthesis: A crucial precursor for creating advanced fluorescent tags and sensors used in bioimaging and cellular analysis.
• Diagnostic Reagents: Employed in the development of colorimetric and fluorometric assay components for clinical and research diagnostics.
• Biological Staining: Used as an intermediate for stains in histology and microscopy to visualize cellular structures.
• Dye Manufacturing: Serves as a key building block in the synthesis of xanthene dyes and other specialized colorants.
• Research & Development: A valuable tool in academic and industrial R&D for photochemical studies and method development.
• Analytical Chemistry: Functions as a standard or reagent in chromatographic and spectroscopic analysis methods.

Basic Information

Product Name	5(6)-Nitrofluorescein
CAS No.	78512-32-0
Molecular Formula	C20H11NO7
Molecular Weight	377.30 g/mol
Synonyms	5(6)-Nitrofluorescein; Nitrofluorescein (mixed isomers); 3',6'-Dihydroxy-5(6)-nitro-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; Fluorescein, 5(6)-nitro-; 5-Nitrofluorescein; 6-Nitrofluorescein; C.I. 45350:2; Nitrofluorescein Isomer Mixture
EINECS	Contact for details

Quality Control

Our 5(6)-Nitrofluorescein is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and industrial applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Keep the container sealed to protect from moisture.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (typical)
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.