Description

3-Hydroxycyclophosphamide is a key intermediate metabolite of the widely used alkylating agent cyclophosphamide. This compound is of significant interest in pharmaceutical research and development, particularly in the study of drug metabolism, mechanism of action, and toxicity profiles. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for analytical reference standards and advanced therapeutic investigations.

Application

• Pharmaceutical Reference Standard: Serves as a critical certified reference material (CRM) for the quantification and qualification of cyclophosphamide and its metabolites in drug substance and product analysis.
• Metabolism and Pharmacokinetic Studies: Used in in vitro and in vivo research to elucidate the metabolic pathways, activation mechanisms, and pharmacokinetic behavior of cyclophosphamide.
• Oncology Research: Employed in biochemical and cellular assays to investigate the detailed mechanism of action, cellular uptake, and resistance profiles of alkylating anticancer agents.
• Toxicology and Safety Assessment: Essential for studying the formation of reactive metabolites, understanding organ-specific toxicity (e.g., urotoxicity), and developing safer therapeutic protocols.
• Analytical Method Development: Used as a standard for developing and validating sensitive analytical methods, such as LC-MS/MS and HPLC, for bioanalysis and impurity profiling.
• Drug-Drug Interaction Studies: Aids in research on how other pharmaceuticals may influence the metabolic activation or deactivation of cyclophosphamide.

Basic Information

Product Name	3-Hydroxycyclophosphamide
CAS No.	78336-02-4
Molecular Formula	C7H15Cl2N2O3P
Molecular Weight	277.09 g/mol
Synonyms	4-Hydroxycyclophosphamide; NSC-26271; 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 4-oxide; Cyclophosphamide Metabolite; Aldophosphamide; 4-Hydroperoxycyclophosphamide (reduced form)
EINECS	Contact for details

Quality Control

Our 3-Hydroxycyclophosphamide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identification by spectroscopic methods (IR, NMR), purity assay by HPLC, and control of related substances and residual solvents. A Certificate of Analysis (COA) detailing all test results and specifications is provided with every shipment to ensure traceability and compliance with your research or quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.