Description

N(10)-Nonylacridine Orange is a cationic, lipophilic acridine dye derivative widely used as a selective fluorescent probe and biological stain. Its core value lies in its ability to interact with and stain specific cellular components, particularly lipids and membranes, due to its nonyl side chain enhancing its lipophilicity. This compound is essential for researchers and industrial laboratories in life sciences, histology, and materials science requiring precise cellular imaging and analysis.

Application

• Fluorescent Staining of Lipids and Membranes: Used as a vital stain for visualizing lipid droplets, intracellular membranes, and lipophilic structures in cell biology and histopathology.
• Cell Viability and Cytotoxicity Assays: Employed in research to assess cell health, membrane integrity, and the effects of pharmaceutical compounds.
• Microscopy and Bioimaging: Serves as a key fluorescent marker in fluorescence microscopy, confocal microscopy, and flow cytometry applications.
• Textile and Polymer Industry: Can be utilized as a dye for synthetic fibers and as a tracer or additive in polymer research.
• Analytical Chemistry Reagent: Acts as a fluorescent probe in developing analytical methods for detecting specific ions or molecules.
• Photodynamic Therapy (PDT) Research: Investigated for its potential as a photosensitizer in preclinical studies for targeted cancer treatments.

Basic Information

Product Name	N(10)-Nonylacridine Orange
CAS No.	78125-98-1
Molecular Formula	C30H35N3
Molecular Weight	437.62 g/mol
Synonyms	Nonyl Acridine Orange; 3,6-Bis(dimethylamino)-10-nonylacridinium; 10-Nonylacridine Orange; Acridine Orange 10-Nonyl Bromide; N10-Nonylacridine Orange; AO-10; Lipophilic Acridine Orange Dye; C.I. 46005 (related base)
EINECS	Contact for details

Quality Control

Our N(10)-Nonylacridine Orange is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometric analysis, and identity confirmation (IR, NMR) to ensure it meets high-purity research-grade standards. A detailed Certificate of Analysis (COA) documenting all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive (store away from light). Keep the container tightly sealed to protect from moisture and contamination.

Specification

Item	Specification
Appearance	Orange to red-orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Fluorescence Properties	Conforms to standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.