Description

Lucifer Yellow is a highly fluorescent, membrane-impermeant dye widely used as a biological tracer and neuronal tracer. Its core value proposition lies in its exceptional photostability and high quantum yield, enabling precise, long-term tracking of cellular structures and connectivity. This product is essential for researchers and professionals in neuroscience, cell biology, and diagnostic assay development, where reliable fluorescent labeling is critical.

Application

• Neuronal Tracing and Microinjection: As a classic gap junction-permeant tracer for studying neuronal circuits and intracellular communication.
• Cell Lineage and Fate Mapping: For long-term tracking of cell migration and differentiation in developmental biology studies.
• Fluorescent Staining of Fixed Tissues: Used in histology and immunohistochemistry for visualizing specific cellular architectures.
• Viability and Cytotoxicity Assays: Employed as an indicator dye in cell-based screening platforms.
• Fluorescence Microscopy and Imaging: A standard fluorescent probe for confocal and two-photon excitation microscopy.
• Ion Channel and Transporter Studies: Utilized in fluorescence-based assays to monitor membrane potential and transport activity.

Basic Information

Product Name	Lucifer Yellow
CAS No.	77944-88-8
Molecular Formula	C₁₃H₉LiN₄O₉S₂
Molecular Weight	457.24 g/mol
Synonyms	Lucifer Yellow CH; Lucifer Yellow CH dilithium salt; 6-Amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid dilithium salt; CI 56059; Acid Yellow 7; LYCH; Lucifer Yellow carbohydrazide
EINECS	Contact for details

Quality Control

Our Lucifer Yellow is produced under stringent quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for fluorescence verification, and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive; prolonged exposure to ambient conditions will degrade performance. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Fluorescence (λex/λem)	~428 nm / ~536 nm
Water Content (KF)	≤ 5.0%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (in Water)	Clear, yellow solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.