Description

4-(n-6-Aminohexylthioureal)Fluorescein is a specialized fluorescent labeling reagent featuring a reactive thiourea group linked to a hexylamine spacer. This compound is engineered for the covalent conjugation to biomolecules, enabling highly sensitive detection and analysis in advanced research applications. It is primarily utilized by researchers and manufacturers in the life sciences sector for developing diagnostic assays, conducting cellular imaging studies, and performing protein or nucleic acid labeling.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to proteins, antibodies, or oligonucleotides for detection in immunoassays (e.g., ELISA, Western blot) and fluorescence in situ hybridization (FISH).
• Cellular and Tissue Imaging: Used as a probe for fluorescence microscopy to visualize specific cellular components or track biomolecules within live or fixed cells.
• Diagnostic Assay Development: Key reagent in the manufacture of research-use-only (RUO) and diagnostic kits for clinical and pharmaceutical testing.
• Flow Cytometry: Conjugation to antibodies or other ligands for the detection and sorting of specific cell populations.
• Bioconjugation Research: Serves as a model compound in developing new conjugation chemistries and studying linker stability.
• Sensor Development: Integration into chemical or biosensor platforms for the detection of specific analytes based on fluorescence signal changes.

Basic Information

Product Name	4-(n-6-Aminohexylthioureal)Fluorescein
CAS No.	77228-88-7
Molecular Formula	C33H30N4O6S
Molecular Weight	610.68 g/mol
Synonyms	4-(6-Aminohexylthioureido)fluorescein; 6-[(Fluorescein-4-yl)thioureido]hexylamine; Fluorescein-4-isothiocyanate hexylamine adduct; FITC-Hexylamine derivative; Aminohexylthiourea-fluorescein; 4-Fluoresceinthiocarbamoylhexane-1,6-diamine; 4-(6-Aminohexylcarbamothioylamino)fluorescein
EINECS	Contact for details

Quality Control

Our 4-(n-6-Aminohexylthioureal)Fluorescein is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing to ensure high purity and consistent performance for sensitive research applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may degrade performance. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Fluorescence Properties	Ex/Em: ~494 nm / ~518 nm (pH >7)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.