Description

4-(n-2-Aminoethylthioureal)Fluorescein is a specialized, reactive derivative of fluorescein, designed for advanced bioconjugation and labeling applications. This compound matters for its ability to form stable covalent bonds with target molecules, enabling precise fluorescent tagging for detection and analysis. It is primarily needed by researchers and manufacturers in the fields of biochemical research, diagnostic assay development, and pharmaceutical analysis.

Application

• Fluorescent Labeling Reagent: For the covalent tagging of proteins, antibodies, peptides, and other biomolecules to create fluorescent probes.
• Bioconjugation Chemistry: Utilized in the synthesis of fluorescent conjugates for use in immunoassays (ELISA, Western Blot) and flow cytometry.
• Molecular Biology Research: As a tracer or detection agent in nucleic acid hybridization studies and in-situ hybridization techniques.
• Diagnostic Kit Development: A key component in the manufacturing of fluorescent-based diagnostic tests and biosensors.
• Cell Imaging and Tracking: For labeling cellular components or whole cells to monitor biological processes via fluorescence microscopy.
• Pharmaceutical Analysis: Used in the development of analytical methods for drug discovery and bio-distribution studies.

Basic Information

Product Name	4-(n-2-Aminoethylthioureal)Fluorescein
CAS No.	77228-87-6
Molecular Formula	C25H21N3O6S
Molecular Weight	491.52 g/mol
Synonyms	4-(2-Aminoethylthioureido)fluorescein; 5-(and-6)-((N-(2-Aminoethyl)thioureido)fluorescein; Aminoethylthiourea-Fluorescein; Fluorescein-5(6)-isothiocyanate isomer derivative; 4-(2-Aminoethyl)thioureidofluorescein; Reactive Fluorescein derivative; 4-Fluoresceinthiocarbamidoethylamine; 5/6-FITC ethylenediamine adduct
EINECS	Contact for details

Quality Control

Our 4-(n-2-Aminoethylthioureal)Fluorescein is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, spectral data, and performance characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	~490-495 nm
Emission (λmax)	~515-520 nm
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.