Description

Azidofluorescein is a versatile, photoactivatable fluorescent dye derivative widely used as a critical labeling reagent in advanced biochemical research. Its core value lies in the azide group, which enables highly specific bioorthogonal conjugation via click chemistry, primarily with alkyne-modified biomolecules. This compound is essential for researchers and manufacturers in the fields of bioconjugation, proteomics, and the development of diagnostic assays and fluorescent probes.

Application

• Bioconjugation & Labeling: Site-specific labeling of proteins, antibodies, nucleic acids, and other biomolecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC).
• Fluorescent Probe Synthesis: A key precursor for creating custom fluorescent sensors, tags, and imaging agents for cellular and in vivo studies.
• Proteomics & Biomarker Discovery: Used in activity-based protein profiling (ABPP) to label and identify enzyme activities within complex biological samples.
• Diagnostic Assay Development: Employed in the fabrication of fluorescent immunoassays, lateral flow tests, and other diagnostic platforms requiring high-sensitivity detection.
• Materials Science: Functionalization of surfaces, nanoparticles, and polymers with fluorescent properties for biosensing and bioimaging applications.
• Chemical Biology Research: Tool for studying biological processes through fluorescence microscopy, flow cytometry, and fluorescence-activated cell sorting (FACS).

Basic Information

Product Name	Azidofluorescein
CAS No.	77162-08-4
Molecular Formula	C21H11N3O5
Molecular Weight	385.33 g/mol
Synonyms	5-Azidofluorescein; 6-Azidofluorescein; Fluorescein azide; 3',6'-Dihydroxy-3-azidospiro[isobenzofuran-1(3H),9'-[9H]xanthene]; 3-Azido-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]; Azido-Fluorescein; Azide-Fluorescein
EINECS	Contact for details

Quality Control

Our Azidofluorescein is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation, to ensure consistent performance and high batch-to-batch reproducibility. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.