Description

5(6)-Azidofluorescein Diacetate is a versatile, cell-permeable fluorescent probe and labeling reagent. Its core value lies in its dual functionality, combining a photoactivatable azide group for bioorthogonal click chemistry with a fluorescein-derived fluorophore for sensitive detection. This compound is essential for researchers and manufacturers in the life sciences sector who require precise, covalent tagging of biomolecules. It is particularly valuable for applications in fluorescence microscopy, flow cytometry, and the development of diagnostic assays.

Application

• Bioorthogonal Labeling: Covalent tagging of proteins, lipids, and glycans via copper-free click chemistry for imaging and tracking studies.
• Live-Cell Imaging: Intracellular labeling and visualization of cellular processes due to its cell-permeable diacetate form, which is hydrolyzed by intracellular esterases to the fluorescent product.
• Flow Cytometry: Fluorescent labeling of cell surface or intracellular targets for high-throughput analysis and cell sorting.
• Diagnostic Assay Development: Used as a key component in the synthesis of fluorescent conjugates for ELISA, lateral flow assays, and other detection platforms.
• Protein & Antibody Conjugation: Site-specific modification of antibodies and other proteins to create fluorescent probes for immunohistochemistry (IHC) and immunofluorescence (IF).
• Material Science: Surface functionalization of polymers and nanoparticles for creating biofunctional materials and sensors.

Basic Information

Product Name	5(6)-Azidofluorescein Diacetate
CAS No.	77162-07-3
Molecular Formula	C26H17N3O7
Molecular Weight	483.43 g/mol
Synonyms	5(6)-Carboxyfluorescein Diacetate N-Succinimidyl Ester, Azide; 5(6)-FAM Azide, Diacetate; 6-Azidofluorescein Diacetate; Azidofluorescein Diacetate; 5-Isomer and 6-Isomer mixture of Azidofluorescein Diacetate; N-Succinimidyl Ester of 5(6)-Carboxyfluorescein Diacetate Azide; Fluorescein-5(6)-azide Diacetate
EINECS	Contact for details

Quality Control

Our 5(6)-Azidofluorescein Diacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic identification (NMR, MS), to ensure consistency and performance for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. Allow the vial to warm to room temperature before opening to minimize moisture condensation. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder or solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95% (sum of isomers)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.