Description

7-Diethylamino-3-[4-(Iodoacetamido)Phenyl]-4-Methylcoumarin is a specialized, highly functionalized coumarin derivative that serves as a critical reagent in advanced biochemical research and diagnostics. Its core value lies in its dual functionality, combining the strong fluorescence of an aminocoumarin scaffold with a reactive iodoacetamide group for selective covalent labeling of thiols, particularly cysteine residues in proteins. This compound is essential for researchers and manufacturers in the fields of bioconjugation, fluorescence labeling, and proteomics, where precise and stable tagging of biomolecules is required. Its design enables the development of sensitive probes for activity-based protein profiling, fluorescence resonance energy transfer (FRET) studies, and the creation of diagnostic assay components.

Application

• Bioconjugation Reagent: Selective covalent labeling of cysteine (thiol) groups in proteins, peptides, and antibodies for creating stable fluorescent conjugates.
• Fluorescent Probe Development: Synthesis of activity-based probes (ABPs) for studying enzyme activity, particularly proteases and other hydrolases, in complex biological samples.
• Protein Labeling & Tracking: Tagging proteins for fluorescence microscopy, cellular imaging, and tracking intracellular localization and dynamics.
• FRET-Based Assays: Serving as a donor or acceptor fluorophore in Förster Resonance Energy Transfer (FRET) pairs for studying molecular interactions and conformational changes.
• Diagnostic Kit Component: Integration into immunoassay and biosensor platforms as a fluorescent label for detection, enhancing sensitivity and specificity.
• Proteomics Research: Used in chemical proteomics for target identification and validation, enabling the study of protein function and drug discovery.
• Material Science: Functionalization of surfaces and nanomaterials with fluorescent properties for sensing applications.

Basic Information

Product Name	7-Diethylamino-3-[4-(Iodoacetamido)Phenyl]-4-Methylcoumarin
CAS No.	76877-34-4
Molecular Formula	C23H23IN2O3
Molecular Weight	502.35 g/mol
Synonyms	7-(Diethylamino)-3-[4-(iodoacetamido)phenyl]-4-methyl-2H-chromen-2-one; 3-[4-(Iodoacetamido)phenyl]-7-(diethylamino)-4-methylcoumarin; Iodoacetamido Coumarin; Thiol-reactive coumarin dye; IAC; 4-Methyl-7-diethylamino-3-[4'-(iodoacetamido)phenyl]coumarin; CM
EINECS	Contact for details

Quality Control

Our 7-Diethylamino-3-[4-(Iodoacetamido)Phenyl]-4-Methylcoumarin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, critical for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and functional testing for labeling efficiency. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all relevant specifications. We support research and development requiring traceable, high-performance biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. For long-term storage, consider keeping the product desiccated at -20°C. Allow the container to reach room temperature before opening to minimize moisture condensation. This compound is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.