Description

Monochlorobimane is a highly reactive, cell-permeable fluorogenic probe used for the selective labeling of thiols, particularly glutathione. This compound matters for researchers and industrial scientists because it enables real-time, non-invasive detection and quantification of cellular thiol status, a critical biomarker for oxidative stress and redox biology. It is essential for professionals in the pharmaceutical, biotechnology, and life science research sectors who require reliable tools for high-throughput screening, cytotoxicity assays, and studying cellular defense mechanisms.

Application

• Glutathione (GSH) Detection and Quantification: Primary application for measuring intracellular glutathione levels via fluorescence spectroscopy or microscopy.
• Oxidative Stress Research: Used as a vital probe to monitor cellular redox state and oxidative stress in various disease models.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify compounds that modulate cellular thiol content or induce oxidative stress.
• Cytotoxicity and Apoptosis Assays: A key reagent for assessing cell viability and early-stage apoptosis linked to thiol depletion.
• Protein Thiol Labeling: Useful for selectively labeling protein thiol groups for analytical or proteomic studies.
• Flow Cytometry: Enables the analysis of thiol distribution and heterogeneity within cell populations.

Basic Information

Product Name	Monochlorobimane
CAS No.	76421-73-3
Molecular Formula	C13H13ClN2O2
Molecular Weight	264.71 g/mol
Synonyms	mBCI; Bimane, 1-chloro-; Chlorobimane; (1-Chloro-2,5-dihydro-1H-imidazo[1,2-a]imidazol-2-yl)methyl Benzoate; 1-Chlorobimane; Glutathione Probe mBCI; ThiolTracker™ Violet (related dye); Cell-permeable thiol-reactive fluorophore
EINECS	Contact for details

Quality Control

Our Monochlorobimane is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant quality management standards to guarantee reliable supply and product integrity for our global B2B clients.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light will degrade its performance. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to pale yellow solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Fluorescence Performance	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.