Description

Diiodofluorescein Isothiocyanate is a reactive fluorescent dye derivative, widely used as a critical labeling reagent in advanced biochemical and diagnostic applications. Its core value lies in its ability to form stable thiourea bonds with primary amines, enabling the specific tagging and detection of biomolecules. This compound is essential for researchers and manufacturers in the fields of immunoassay development, fluorescence microscopy, and flow cytometry, where high-sensitivity detection is paramount.

Application

• Fluorescent Labeling of Antibodies and Proteins: Conjugation for use in immunofluorescence (IF) and immunohistochemistry (IHC) applications.
• Flow Cytometry Probe Development: Tagging of cell surface markers or intracellular targets for high-throughput cellular analysis.
• Fluorescence In Situ Hybridization (FISH): Preparation of labeled nucleic acid probes for genetic and cytogenetic testing.
• Diagnostic Kit Manufacturing: A key component in the production of lateral flow assays and ELISA kits for clinical diagnostics.
• Biomolecular Imaging: Used in fluorescence microscopy for tracking and visualizing specific proteins or cellular structures.
• Research Reagent: Essential tool in academic and pharmaceutical R&D for studying protein interactions, localization, and expression.

Basic Information

Product Name	Diiodofluorescein Isothiocyanate
CAS No.	76391-89-4
Molecular Formula	C21H9I2NO5S
Molecular Weight	689.17 g/mol
Synonyms	5(6)-Diiodofluorescein Isothiocyanate; 5(6)-Diiodofluorescein-5-isothiocyanate; 5(6)-Diiodofluorescein Isothiocyanate Isomer Mixture; Diiodofluorescein-5-isothiocyanate; DIFITC; 4',5'-Diiodofluorescein-5-isothiocyanate; 4',5'-Diiodofluorescein isothiocyanate; 2-(3',6'-Diiodo-3-oxo-6-(isothiocyanato)-3H-spiro[isobenzofuran-1,9'-xanthene]-5(or 6)-yl)benzoic acid
EINECS	Contact for details

Quality Control

Our Diiodofluorescein Isothiocyanate is produced under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and functional performance validation in standard conjugation protocols. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant after each use to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Isomer Mixture)
Extinction Coefficient	Contact for details
Fluorescence Emission	~525 nm (upon conjugation, pH dependent)
Water Content (KF)	≤ 3.0 %
Functional Performance	Conjugation efficiency confirmed vs. standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.