Description

5(6)-Carboxytetramethylrhodamine n-Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent, widely recognized as a superior alternative to TRITC. Its core value lies in its exceptional photostability, high fluorescence quantum yield, and water solubility, enabling the creation of bright, stable conjugates for sensitive detection. This compound is essential for researchers and manufacturers in the life sciences and diagnostics sectors who require reliable fluorescent probes for advanced applications.

Application

• Fluorescent Labeling of Biomolecules: Primary reagent for covalently labeling antibodies, proteins, peptides, and oligonucleotides via primary amine groups (lysine residues or N-termini).
• Flow Cytometry & Fluorescence-Activated Cell Sorting (FACS): Used to prepare bright antibody conjugates for cell surface marker detection and analysis.
• Fluorescence Microscopy & Immunohistochemistry (IHC): Creates stable, photobleach-resistant probes for high-resolution cellular and tissue imaging.
• Fluorescence In Situ Hybridization (FISH): Labels nucleic acid probes for chromosome and gene mapping studies.
• Diagnostic Assay Development: Key component in developing fluorescent immunoassays (FLISA), lateral flow tests, and other clinical diagnostic kits.
• Bioconjugation & Chemical Biology: Used in synthesizing fluorescently tagged small molecules, polymers, and nanoparticles for tracking and targeting studies.

Basic Information

Product Name	5(6)-Carboxytetramethylrhodamine n-Succinimidyl Ester
CAS No.	75929-56-5
Molecular Formula	C29H26N2O7
Molecular Weight	514.53 g/mol
Synonyms	5(6)-TAMRA, SE; 5(6)-Carboxy-tetramethylrhodamine N-succinimidyl ester; TAMRA, SE isomer mixture; 5(6)-Carboxy-X-rhodamine, SE; 6-TAMRA NHS ester; Tetramethylrhodamine-5(6)-isothiocyanate isomer mixture; 5(6)-TAMRA, succinimidyl ester; Mixed isomers of 5- and 6-carboxytetramethylrhodamine N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxytetramethylrhodamine n-Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and isomer ratio is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0% (Isomer mixture)
Absorption Maximum (λmax)	540 - 555 nm
Emission Maximum	565 - 580 nm
Extinction Coefficient (ε)	~90,000 cm-1M-1
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; moderately soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.