Description

Fluoresceinamine Maleic Acid Monoamide is a specialized fluorescent labeling reagent that combines the high-quantum-yield properties of fluorescein with a reactive maleimide group. This bifunctional nature is critical for creating stable, site-specific conjugates with thiol-containing biomolecules, enabling advanced research and diagnostic applications. It is an essential tool for scientists and manufacturers in the fields of bioconjugation, proteomics, and immunoassay development, where precise and stable fluorescent tagging is required.

Application

• Bioconjugation and Protein Labeling: Site-specific covalent attachment to cysteine residues in proteins and antibodies for fluorescence-based studies.
• Immunoassay Development: Preparation of fluorescently-labeled antibodies and antigens for use in ELISA, lateral flow assays, and other diagnostic platforms.
• Proteomics and Biomarker Research: Tagging of peptides and proteins for detection, purification, and analysis via fluorescence detection methods.
• Fluorescent Probe Synthesis: Serving as a key intermediate in the synthesis of advanced molecular probes and sensors for cellular imaging.
• Drug Delivery Research: Conjugation to drug carriers or therapeutic molecules to track distribution and cellular uptake.
• Material Science: Functionalization of nanoparticles and polymeric materials with fluorescent markers for tracking and characterization.

Basic Information

Product Name	Fluoresceinamine Maleic Acid Monoamide
CAS No.	75900-74-2
Molecular Formula	C25H16N2O7
Molecular Weight	456.41 g/mol
Synonyms	5-((3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanamido)methyl)fluorescein; Fluorescein-5-maleimide; 5-Fluorescein Maleimide; 5-FAM Maleimide; Fluorescein Maleimide; 5-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)amino)fluorescein; 1-(3-((6-Hydroxy-3-oxo-3H-xanthen-9-yl)methylamino)propyl)-1H-pyrrole-2,5-dione
EINECS	Contact for details

Quality Control

Every batch of Fluoresceinamine Maleic Acid Monoamide is manufactured and tested under a strict quality management system. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive life science applications. A detailed Certificate of Analysis (COA) is supplied with each shipment, confirming identity, purity (typically ≥95% by HPLC), and performance in conjugation reactions.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	~ 492 nm
Emission (λmax)	~ 518 nm
Extinction Coefficient	~ 80,000 cm-1M-1
Water Content (KF)	≤ 2.0%
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.