Description

1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate is a specialized, high-purity cyanine dye salt, widely recognized for its exceptional photostability and intense fluorescence in the near-infrared (NIR) region. This compound is critical for applications requiring deep-tissue imaging and high-sensitivity detection due to its long-wavelength emission and excellent quantum yield. It is an essential reagent for researchers and manufacturers in the biotechnology, medical diagnostics, and advanced materials sectors.

Application

• Fluorescent Labeling & Probes: Used as a covalent or non-covalent label for biomolecules (proteins, nucleic acids, lipids) in fluorescence microscopy, flow cytometry, and immunoassays.
• Bioimaging & Diagnostics: A key component in developing contrast agents and probes for in vivo and in vitro imaging, particularly for NIR fluorescence imaging in preclinical research.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer due to its ability to generate reactive oxygen species upon light activation.
• Materials Science: Incorporated into organic light-emitting diodes (OLEDs), solar cells, and other optoelectronic devices as a functional dye.
• Sensor Development: Employed in the fabrication of chemical and biological sensors that utilize fluorescence quenching or enhancement mechanisms.
• Cell Membrane Staining: Utilized for labeling and tracking cell membranes in live-cell imaging due to its lipophilic didodecyl chains.

Basic Information

Product Name	1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate
CAS No.	75664-01-6
Molecular Formula	C53H85ClN2O4
Molecular Weight	849.70 g/mol
Synonyms	DiD Perchlorate; DiIC12(3) Perchlorate; 1,1'-Didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; DiI (C12) Perchlorate; 4,5-Bis(dodecyl)-3H,3'H-indolium, 2-[2-[2-chloro-3-[(1,3-dihydro-3,3-dimethyl-1-(dodecyl)-2H-indol-2-ylidene)ethylidene]-1-cyclopenten-1-yl]ethenyl]-3,3-dimethyl-1-dodecyl-, perchlorate; DiIC12(3); DID (C12) Dye; Lipophilic Tracer DiD
EINECS	Contact for details

Quality Control

Every batch of our 1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate is manufactured under strict quality management systems. We employ advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure high chemical purity, identity confirmation, and batch-to-batch consistency, meeting the rigorous demands of research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence profiles, and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to warm to room temperature before opening to minimize moisture condensation. This material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Dark green to blue-black solid or crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95.0% (Area %)
Absorption Maximum (λmax)	644 nm ± 3 nm (in methanol)
Emission Maximum	663 nm ± 3 nm (in methanol)
Extinction Coefficient (ε)	≥ 200,000 L·mol⁻¹·cm⁻¹ (at λmax)
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.