Description

5-(N'-[2-Aminoethyl]Thioureidofluorescein) is a specialized, reactive derivative of fluorescein designed for covalent conjugation, primarily used as a fluorescent labeling reagent. This compound matters for researchers and manufacturers who require a highly sensitive and stable fluorescent tag with an amine-reactive thiourea linkage for biomolecule analysis. It is essential for professionals in the life sciences, diagnostics, and pharmaceutical development sectors who need precise, covalent fluorescent labeling of proteins, antibodies, and other amine-containing molecules.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and quantification assays.
• Flow Cytometry & Cell Imaging: Preparation of fluorescent probes for cell surface marker staining and intracellular tracking in research and diagnostics.
• Immunoassay Development: Creation of fluorescent conjugates for ELISA, Western blotting, and other immunoassay platforms.
• Bioconjugation & Probe Synthesis: Serving as a key intermediate in the synthesis of advanced fluorescent probes and sensor molecules.
• Diagnostic Kit Manufacturing: Used in the production of fluorescent-based diagnostic tests and kits for clinical and research use.
• Protein Interaction Studies: Labeling proteins to study protein-protein interactions, localization, and dynamics using fluorescence techniques.

Basic Information

Product Name	5-(N'-[2-Aminoethyl]Thioureidofluorescein)
CAS No.	75453-82-6
Molecular Formula	C25H21N3O6S
Molecular Weight	491.52 g/mol
Synonyms	5-(2-Aminoethyl)thioureidofluorescein; Fluorescein-5-thiosemicarbazide; 5-Isothiocyanatofluorescein amine derivative; 5-FITC ethylenediamine adduct; 5-(2-Aminoethyl)thiocarbamoylfluorescein; Reactive Fluorescein Isothiocyanate derivative; 5-ATT Fluorescein; 5-(N-(2-Aminoethyl)carbamothioyl)fluorescein
EINECS	Contact for details

Quality Control

Our 5-(N'-[2-Aminoethyl]Thioureidofluorescein) is produced under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, consider desiccation or storage under inert atmosphere to maintain stability and reactivity. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λ max)	~490-495 nm
Emission (λ max)	~515-520 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.