Description

Fluorescein Thiosemicarbazide is a high-purity, reactive derivative of fluorescein, designed for advanced conjugation and labeling applications. This compound is valued for its ability to introduce a thiosemicarbazide functional group, enabling highly specific coupling to aldehydes and ketones under mild conditions. It is essential for researchers and manufacturers in the fields of bioanalytical chemistry, diagnostics, and materials science who require precise, fluorescent tagging of biomolecules and polymers.

Application

• Bioconjugation and Protein Labeling: Selective tagging of glycoproteins and other carbonyl-containing biomolecules for fluorescence microscopy, flow cytometry, and immunoassays.
• Diagnostic Probe Development: Synthesis of fluorescent sensors and probes for the detection of specific analytes, pathogens, or enzymatic activity.
• Polymer and Materials Science: Functionalization of surfaces, nanoparticles, and hydrogels to create fluorescently tagged materials for tracking and imaging studies.
• Chemical Biology Research: Tool compound for studying biomolecular interactions, localization, and dynamics in live-cell and fixed-cell imaging.
• High-Throughput Screening (HTS): Development of fluorescent assays for drug discovery and biochemical pathway analysis.
• Chromatography and Electrophoresis: Preparation of fluorescent markers and standards for gel and capillary-based separation techniques.

Basic Information

Product Name	Fluorescein Thiosemicarbazide
CAS No.	75323-82-9
Molecular Formula	C21H15N3O5S
Molecular Weight	421.43 g/mol
Synonyms	5(6)-Carboxyfluorescein Thiosemicarbazide; Fluorescein-5(6)-Thiosemicarbazide; 3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carbothiohydrazide; 5(6)-FITSC; Fluorescein Isothiocyanate Thiosemicarbazide (common precursor name); FISC; 5/6-FAM Thiosemicarbazide
EINECS	Contact for details

Quality Control

Our Fluorescein Thiosemicarbazide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant or under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	~490 nm
Emission (λmax)	~515 nm
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.