Description

Nonyl Acridine Orange is a specialized cationic dye and fluorescent probe derived from acridine orange, modified with a nonyl side chain to enhance its lipophilicity and membrane-binding properties. This compound is critical for researchers and industrial developers requiring precise, high-contrast staining of acidic organelles, particularly lysosomes, in live and fixed cell studies. It is an essential tool for professionals in cell biology research, diagnostic assay development, and flow cytometry applications, where reliable fluorescence and specific organelle targeting are paramount.

Application

• Live-Cell Lysosomal Staining: Primary application for visualizing and tracking lysosome morphology, motility, and pH in real-time using fluorescence microscopy.
• Flow Cytometry Analysis: Used as a fluorescent marker to identify, count, and analyze lysosome-rich cell populations or to assess lysosomal function.
• Apoptosis and Cell Viability Assays: Employed in research to investigate mechanisms of cell death, where lysosomal membrane permeabilization is a key event.
• Diagnostic Reagent Formulation: Incorporated into kits and reagents for clinical and research diagnostics focused on lysosomal storage disorders or cellular health.
• Photodynamic Therapy (PDT) Research: Investigated as a potential photosensitizer due to its light-absorbing properties and specific subcellular localization.
• Material Science & Polymer Staining: Applied in niche areas for staining certain polymers or materials to study porosity and degradation under fluorescent light.

Basic Information

Product Name	Nonyl Acridine Orange
CAS No.	75168-11-5
Molecular Formula	C31H41N3
Molecular Weight	455.68 g/mol
Synonyms	NAO; Nonyl Acridine Orange dye; 3,6-Bis(dimethylamino)-10-nonylacridinium; 10-Nonylacridine Orange; Lysosomal probe NAO; Acridine Orange C9; Acridine Orange nonyl derivative; Fluorescent lysosomal marker
EINECS	Contact for details

Quality Control

Every batch of Nonyl Acridine Orange is manufactured and analyzed under strict quality protocols to ensure high purity, consistent fluorescence performance, and batch-to-batch reproducibility. Our quality system includes comprehensive analytical testing via HPLC, NMR, and mass spectrometry to confirm chemical identity and purity. A detailed Certificate of Analysis (COA), documenting all critical quality attributes, is provided with each shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage under inert atmosphere, consider aliquoting and storing at -20°C. Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Orange to dark red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Fluorescence Properties	Ex/Em: ~490/525 nm (bound form)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.