Description

3-Azidoethidium is a specialized azide-functionalized ethidium derivative, a vital chemical tool for advanced biochemical research and diagnostic development. Its core value lies in its ability to act as a photoaffinity labeling agent and a fluorescent probe, enabling the study of nucleic acid interactions and protein binding sites. This compound is essential for researchers and developers in the fields of molecular biology, pharmacology, and diagnostic assay development, particularly for investigating DNA structure and function.

Application

• Photoaffinity Labeling: Used as a covalent cross-linking agent to study nucleic acid-protein interactions and identify binding sites.
• Fluorescent Probing: Acts as a sensitive fluorescent marker for DNA and RNA in gel electrophoresis and other analytical techniques.
• Biochemical Research: A key reagent for investigating the mechanisms of intercalating agents and their effects on nucleic acid conformation.
• Diagnostic Development: Employed in the research and development of novel diagnostic assays and kits that target specific nucleic acid sequences.
• Antiviral/Antibacterial Studies: Utilized in research to evaluate potential compounds that interact with microbial DNA or RNA.
• Academic & Industrial R&D: Serves as a critical tool in university laboratories and biotech/pharmaceutical R&D departments for fundamental and applied life science research.

Basic Information

Product Name	3-Azidoethidium
CAS No.	74444-55-6
Molecular Formula	C21H20N6
Molecular Weight	356.43 g/mol
Synonyms	3-Azidoethidium Bromide; Ethidium Monoazide; 3-Azido-7,8-dimethylphenanthridin-5-ium; Azidoethidium; Phenanthridinium, 3-azido-7,8-dimethyl-; Photoactivatable Ethidium Derivative
EINECS	Contact for details

Quality Control

Our 3-Azidoethidium is produced and tested under stringent quality management protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry, and well-ventilated area. Keep the container sealed to protect this light-sensitive compound from degradation.

Specification

Item	Specification
Appearance	Red to brown solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.