Description

4-Azidophenyl Isothiocyanate is a specialized bifunctional chemical reagent that combines a photoreactive azido group with an isothiocyanate moiety, enabling versatile covalent bonding strategies. This compound is critical for researchers and manufacturers requiring precise, stable conjugation in advanced material and life science applications. It is primarily utilized in the development of photoaffinity labels, surface modification, polymer cross-linking, and the synthesis of bioactive probes for pharmaceutical and diagnostic research.

Application

• Photoaffinity Labeling: A key reagent for creating covalent bonds with biological targets upon UV irradiation, essential for studying protein-ligand interactions and mapping binding sites.
• Bioconjugation & Probe Synthesis: Used to attach reporter molecules (e.g., fluorophores, biotin) to proteins, antibodies, or nanoparticles via its reactive isothiocyanate group, which couples with primary amines.
• Surface Functionalization: Employed to modify material surfaces (e.g., gold, glass, polymers) with azide groups for subsequent "click chemistry" reactions, enabling the creation of biosensors and advanced coatings.
• Polymer & Hydrogel Cross-linking: Acts as a cross-linker in the synthesis of smart polymers and hydrogels, where the azide group can be activated to form covalent networks.
• Pharmaceutical Intermediate: Serves as a building block in the synthesis of more complex molecules for drug discovery and development programs.
• Diagnostic Assay Development: Facilitates the stable immobilization of capture molecules on solid supports for ELISA, microarray, and lateral flow assay platforms.

Basic Information

Product Name	4-Azidophenyl Isothiocyanate
CAS No.	74261-65-7
Molecular Formula	C7H4N4S
Molecular Weight	176.20 g/mol
Synonyms	p-Azidophenyl Isothiocyanate; 4-Azido-1-isothiocyanatobenzene; 1-Azido-4-isothiocyanatobenzene; 4-Azidophenyl Mustard Oil; 4-Azidophenyl ITC; APITC; 4-Isothiocyanatophenyl azide; (4-Isothiocyanatophenyl)azide
EINECS	Contact for details

Quality Control

Every batch of 4-Azidophenyl Isothiocyanate is manufactured and tested under strict quality management systems to ensure high purity and batch-to-batch consistency. Our products undergo rigorous quality testing, including HPLC, NMR, and FT-IR analysis, to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at 2-8°C. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may lead to decomposition. Keep container tightly sealed under an inert atmosphere (e.g., nitrogen or argon) for long-term storage.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.